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Expression and Purification of N-Terminally His-Tagged Recombinant Type III Secretion Proteins

Travis D Alvine1, Patrick Osei-Owusu2, Danielle L Jessen Condry3

  • 1Department of Biomedical Sciences, University of North Dakota, 1301 North Columbia Road, Stop 9037, Grand Forks, ND, 58203, USA.

Methods in Molecular Biology (Clifton, N.J.)
|November 13, 2016
PubMed

Keywords:
Blunt-end PCR cloningHis-tagProtein expressionProtein purificationpET200 vector

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