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Related Experiment Video

Updated: Mar 12, 2026

Histological-Based Stainings Using Free-Floating Tissue Sections

Histological-Based Stainings Using Free-Floating Tissue Sections

Published on: August 25, 2020

Histopathology.

Renate Kain¹

¹Clinical Institute of Pathology, Medical University of Vienna, Währinger Gürtel 18-20/5P, Vienna, 1090, Austria. renate.kain@meduniwien.ac.at.

Methods in Molecular Biology (Clifton, N.J.)

|November 13, 2016

Summary

Histological stains like Gomori methenamine silver (GMS) and Periodic acid-Schiff (PAS) are crucial for detecting fungal elements in patient tissues. These methods complement microbial cultures, aiding in diagnosing fungal infections and assessing tissue invasion.

Area of Science:

Medical Mycology
Histopathology
Diagnostic Microbiology

Background:

Fungal elements in patient specimens offer critical diagnostic information.
Fungi appear on histological sections of skin/mucosa or as emboli occluding vessels in immunocompromised patients or autopsy tissues.
Microbial culture is standard for diagnosing fungal infections, but histomorphology is essential for identifying unsuspected infections or evaluating tissue invasion.

Purpose of the Study:

To highlight the importance of histological stains in fungal detection.
To explain the mechanism of common fungal stains.
To emphasize the complementary role of histomorphology alongside microbial cultures.

Main Methods:

Utilizing oxidizing reagents to create aldehyde binding sites on fungal cell wall polysaccharides (1,2-glycol groups).

Keywords:

Histochemistry PAS stain Schiffs’ reaction Silver stain

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Employing Schiff's reagent or Silver ions for staining.

Applying Gomori methenamine silver (GMS) and Periodic acid-Schiff (PAS) stains or their modifications on tissue and cytology specimens.

Main Results:

Histological stains effectively identify fungal elements in various tissue types.
Specific stains enable the detection of fungal infections not apparent through other methods.
GMS and PAS stains are widely used and effective for visualizing fungi in histopathology and cytology.

Conclusions:

Histological staining is a fundamental technique for detecting and characterizing fungal elements in patient samples.
Gomori methenamine silver (GMS) and Periodic acid-Schiff (PAS) are indispensable stains for diagnosing fungal infections.
Histomorphology, particularly with GMS and PAS, complements microbial cultures for comprehensive fungal diagnosis.