Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

NN15-017 promotes human pluripotent stem cell proliferation and generation.

Regenerative therapy·2025
Same author

Acetylation of lysine 49 on Ctnnb1 drives naïve pluripotency in murine stem cells by modulating Nanog function.

PNAS nexus·2025
Same author

Inhibition of N-myristoyltransferase in pluripotent stem cells promotes the naive state in mice and elicits trophectoderm and primitive endoderm markers in humans.

Stem cell reports·2025
Same author

Secondary Publication: Proposal for Points of Consideration for Pluripotent Stem Cell Culture.

In vitro cellular & developmental biology. Animal·2024
Same author

Establishment of the Embryo-derived Stem (ES) Cell Lines from Mouse Blastocysts: Effects of the Feeder Cell Layer.

Development, growth & differentiation·2023
Same author

Radial Columnar Patches in the Chimeric Cerebral Cortex Visualized by Use of Mouse Embryonic Stem Cells Expressing β-Galactosidase: (mouse chimera/ES cell/cerebral cortex/β-galactosidase).

Development, growth & differentiation·2023

Related Experiment Video

Updated: Mar 12, 2026

A cGMP-applicable Expansion Method for Aggregates of Human Neural Stem and Progenitor Cells Derived From Pluripotent Stem Cells or Fetal Brain Tissue
09:37

A cGMP-applicable Expansion Method for Aggregates of Human Neural Stem and Progenitor Cells Derived From Pluripotent Stem Cells or Fetal Brain Tissue

Published on: June 15, 2014

14.2K

Slow Cooling Cryopreservation Optimized to Human Pluripotent Stem Cells.

Takamichi Miyazaki1, Hirofumi Suemori2

  • 1Institute for Integrated Cell-Material Sciences, Kyoto University, Kyoto, Japan.

Advances in Experimental Medicine and Biology
|November 13, 2016
PubMed
Summary

Human pluripotent stem cells (hPSCs) cryopreservation using slow cooling has been challenging due to low cell viability. This review explores reasons for failure and optimizes methods for successful hPSC storage.

Keywords:
CryopreservationHuman embryonic stem cellHuman induced pluripotent stem cellsHuman pluripotent stem cellSlow-coolingVitrification

More Related Videos

Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis
08:27

Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis

Published on: July 24, 2014

12.9K
Freezing Human ES Cells
08:00

Freezing Human ES Cells

Published on: October 12, 2006

24.1K

Related Experiment Videos

Last Updated: Mar 12, 2026

A cGMP-applicable Expansion Method for Aggregates of Human Neural Stem and Progenitor Cells Derived From Pluripotent Stem Cells or Fetal Brain Tissue
09:37

A cGMP-applicable Expansion Method for Aggregates of Human Neural Stem and Progenitor Cells Derived From Pluripotent Stem Cells or Fetal Brain Tissue

Published on: June 15, 2014

14.2K
Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis
08:27

Alternative Cultures for Human Pluripotent Stem Cell Production, Maintenance, and Genetic Analysis

Published on: July 24, 2014

12.9K
Freezing Human ES Cells
08:00

Freezing Human ES Cells

Published on: October 12, 2006

24.1K

Area of Science:

  • Stem Cell Biology
  • Cryobiology
  • Cell Preservation

Background:

  • Human pluripotent stem cells (hPSCs) offer vast potential for regenerative medicine.
  • Effective cryopreservation is crucial for hPSC applications, enabling storage and transport.
  • Traditional slow cooling methods, successful for other cell types, have yielded poor viability in hPSCs.

Purpose of the Study:

  • Investigate the underlying reasons for the failure of slow cooling in hPSC cryopreservation.
  • Identify factors contributing to cell death during freeze-thawing stages.
  • Summarize advancements and discuss optimization strategies for hPSC cryopreservation.

Main Methods:

  • Review of existing literature on hPSC cryopreservation techniques.
  • Analysis of factors influencing cell viability during cryopreservation, including colony state.
  • Discussion of conventional cryopreservation methods applicable to hPSCs.

Main Results:

  • hPSCs are difficult to cryopreserve using standard slow cooling, often resulting in low cell viability.
  • Cell death during freeze-thawing is a significant issue, with the pre-cryopreservation colony state being a major contributing factor.
  • Conventional cryopreservation methods can be optimized for hPSC storage without specialized equipment.

Conclusions:

  • Understanding the specific challenges of hPSC cryopreservation is key to improving viability.
  • Optimizing slow cooling protocols, considering factors like colony state, can enable successful hPSC preservation.
  • Advances allow for effective hPSC cryopreservation using conventional methods, facilitating their broader use.