Mercury detection based on label-free and isothermal enzyme-free amplified fluorescence platform

A novel and convenient biosensor for Mercury (II) detection was developed based on toehold-mediated strand displacement isothermal enzyme-free amplification (EFA) technology and label-free fluorescence platform using Sybr Green Ι (SG) and graphene oxide (GO). The method is highly sensitive and selective, and the logarithmically related Hg²⁺ linearity range is from 0.1 to 50nM with a detection limit 0.091nM. Moreover, our strategy is simple, enzyme-free, and inexpensive and can be applied to detect spiked Hg²⁺ in environmental water samples with good recovery and accuracy, which demonstrates that the biosensor has a good potential in the environment surveys in the future.