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Development of highly transferable microsatellites for Panax ginseng (Araliaceae) using whole-genome data
Peng Jiang1, Feng-Xue Shi2, Ya-Ling Li1
1Key Laboratory of Molecular Epigenetics of the Ministry of Education, Northeast Normal University, Changchun 130024, People's Republic of China.
Premise Of The Study:
Highly transferable expressed sequence tag (EST) microsatellites were developed for Panax ginseng (Araliaceae), one of the most celebrated traditional Chinese medicines and an endangered species in East Asia, using whole-genome data.
Methods And Results:
Twenty-one EST microsatellites were characterized from next-generation sequencing and were composed of di- and trinucleotide repeats. Polymorphisms and genetic diversity were evaluated for 45 accessions of three ginseng landraces. The number of alleles for each locus ranged from one to five among the landraces, and the polymorphism information content varied from 0.0000 to 0.6450. These microsatellites were also tested for congeneric amplification with P. notoginseng, P. stipuleanatus, P. quinquefolius, P. bipinnatifidus, and the closely related species Aralia elata.
Conclusions:
These novel EST-derived microsatellite markers will facilitate further population genetic studies of the genera Panax and Aralia.
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