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Published on: January 22, 2018
Microsatellite primers for the rare shrub Acacia adinophylla (Fabaceae)
Paul G Nevill1, Grant Wardell-Johnson2
1Australian Research Council Centre for Mine Restoration, Department of Environment and Agriculture, Curtin University, Bentley 6102, Western Australia, Australia; School of Plant Biology, University of Western Australia, Nedlands 6009, Western Australia, Australia.
Premise Of The Study:
Microsatellite primers were developed for the rare shrub Acacia adinophylla (Fabaceae) to assess genetic diversity and its spatial structuring.
Methods And Results:
Shotgun sequencing on an Illumina MiSeq produced 6,372,575 reads. Using the QDD pipeline, we designed 60 primer pairs, which were screened using PCR. Seventeen loci were developed, of which 12 loci were identified that were polymorphic, amplified reliably, and could be consistently scored. These loci were then screened for variation in individuals from three populations. The number of alleles observed for these 12 loci ranged from three to 18 and expected heterozygosity ranged from 0.13 to 0.85.
Conclusions:
These markers will enable the quantification of genetic impact of proposed mining activities on the short-range endemic Acacia adinophylla.
