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Updated: Mar 12, 2026
Medium-scale Preparation of Drosophila Embryo Extracts for Proteomic Experiments
Published on: May 30, 2017
SWATH-MS data of Drosophila melanogaster proteome dynamics during embryogenesis
Bertrand Fabre1, Dagmara Korona2, Daniel J H Nightingale1
1Cambridge Centre for Proteomics, Department of Biochemistry, University of Cambridge, Cambridge, U.K; Department of Biochemistry, University of Cambridge, Cambridge, U.K; Cambridge Systems Biology Centre, University of Cambridge, Cambridge, U.K.
Abstract:
Embryogenesis is one of the most important processes in the life of an animal. During this dynamic process, progressive cell division and cellular differentiation are accompanied by significant changes in protein expression at the level of the proteome. However, very few studies to date have described the dynamics of the proteome during the early development of an embryo in any organism. In this dataset, we monitor changes in protein expression across a timecourse of more than 20 h of Drosophila melanogaster embryonic development. Mass-spectrometry data were produced using a SWATH acquisition mode on a Sciex Triple-TOF 6600. A spectral library built in-house was used to analyse these data and more than 1950 proteins were quantified at each embryonic timepoint. The files presented here are a permanent digital map and can be reanalysed to test against new hypotheses. The data have been deposited with the ProteomeXchange Consortium with the dataset identifier PRIDE: PXD0031078.
