Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Soluble CD8 during T cell activation.

B E Tomkinson1, M C Brown, S H Ip

  • 1Department of Pediatrics, University of Massachusetts Medical School, Worcester 01655.

Journal of Immunology (Baltimore, Md. : 1950)
|April 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The role of Lin28b in myeloid and mast cell differentiation and mast cell malignancy.

Leukemia·2015
Same author

On the antioxidant properties of erythropoietin and its association with the oxidative-nitrosative stress response to hypoxia in humans.

Acta physiologica (Oxford, England)·2014
Same author

Robotic training and kinematic analysis of arm and hand after incomplete spinal cord injury: a case study.

IEEE ... International Conference on Rehabilitation Robotics : [proceedings]·2012
Same author

Putative hepatoprotective effects of coffee.

Alimentary pharmacology & therapeutics·2007
Same author

Is homocysteine an iron-dependent cardiovascular risk factor?

Kidney international·2006
Same author

In utero HIV infection in pregnancies complicated by tuberculosis in Durban, South Africa.

Archives of disease in childhood. Fetal and neonatal edition·2004

Researchers developed a new assay to measure soluble CD8, a marker released by activated CD8+ T cells. Elevated soluble CD8 levels in infectious mononucleosis patients indicate its potential for monitoring T cell responses in disease.

Area of Science:

  • Immunology
  • Cell Biology

Background:

  • CD8 antigen (Ag) is a key surface marker for cytotoxic and suppressor T cells.
  • Recent findings indicate CD8-positive cells release a soluble form of the CD8 Ag.
  • The clinical significance of soluble CD8 release requires further investigation.

Purpose of the Study:

  • To develop a quantitative assay for soluble CD8.
  • To investigate the release of soluble CD8 during lymphocyte activation.
  • To assess the potential of soluble CD8 as a biomarker for CD8+ T cell activation in disease.

Main Methods:

  • Development of a sandwich monoclonal enzyme immunoassay for soluble CD8 quantitation.
  • In vitro studies involving PHA or anti-CD3 mAb-mediated T cell activation.
  • In vivo analysis of serum from patients with EBV-induced infectious mononucleosis (IM) and healthy controls.

Related Experiment Videos

Main Results:

  • Soluble CD8 was released upon lymphocyte activation in vitro.
  • Serum soluble CD8 levels were significantly elevated in IM patients compared to controls.
  • Soluble CD8 levels strongly correlated with activated CD8+/HLA-DR+ T cells and mirrored disease course.

Conclusions:

  • The developed immunoassay effectively quantifies soluble CD8.
  • Soluble CD8 release is linked to CD8+ T cell activation.
  • Soluble CD8 shows promise as a biomarker for monitoring T cell involvement in pathological conditions.