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Characterization of the cervical mucus plug in mares
S C Loux1, K E Scoggin1, M H T Troedsson1
1Department of Veterinary ScienceUniversity of Kentucky, Lexington, Kentucky, USA.
Reproduction (Cambridge, England)
|November 16, 2016
Summary
The equine cervical mucus plug (CMP) contains immune-supporting proteins and originates from the cervix, not the endometrium. Its composition changes throughout pregnancy to protect the mare and fetus from infection.
Area of Science:
- Reproductive biology
- Equine reproduction
- Proteomics
Background:
- The equine cervical mucus plug (CMP) is crucial for maintaining pregnancy by preventing microbial invasion.
- Its precise composition and origin remain largely uncharacterized.
Purpose of the Study:
- To elucidate the proteomic composition and origin of the equine CMP during late gestation.
- To understand the role of CMP composition in equine reproductive health.
Main Methods:
- Proteomic analysis of CMPs from mares using nano-LC-MS/MS.
- Histological examination with H&E and PAS staining.
- Quantitative PCR (qPCR) to analyze mRNA expression in cervical and endometrial tissues.
Main Results:
- The CMP comprises a complex protein mixture with significant immune functions.
- Proteins previously thought to be endometrial are produced by the cervical mucosa.
- CMP composition is dynamically regulated throughout the estrous cycle and gestation.
Conclusions:
- The equine CMP is primarily derived from the cervical mucosa.
- Its complex and regulated composition provides both structural and physiological barriers against infection.
- Understanding CMP composition offers insights into maintaining equine pregnancy.
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