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Assessing the effects of threonyl-tRNA synthetase on angiogenesis-related responses

Adam C Mirando1, Khadar Abdi1, Peibin Wo1

  • 1Department of Pharmacology, College of Medicine, University of Vermont, Burlington, VT 05405, United States.

Methods (San Diego, Calif.)
|November 17, 2016
PubMed

Insights

Researchers explored assays for aminoacyl-tRNA synthetases in angiogenesis. This review details methods to assess threonyl-tRNA synthetase

Area of Science:

  • Biochemistry and Molecular Biology
  • Cell Biology
  • Physiology

Background:

  • Recent studies link aminoacyl-tRNA synthetases to angiogenesis regulation.
  • Understanding these enzymes' roles in blood vessel formation is crucial.
  • Reliable assays are needed to study these functions.

Purpose of the Study:

  • To review and detail in vitro and in vivo assays for assessing angiogenesis functions of enzymes.
  • To focus on methods for evaluating threonyl-tRNA synthetase's role in angiogenesis.
  • To discuss assay analysis, quantification, advantages, and limitations.

Main Methods:

  • In vitro assays: endothelial cell migration and tube formation assays.
  • In vivo assays: chorioallantoic membrane assay and tumor vascularization assay.
  • Review of theoretical frameworks, analytical techniques, and quantification strategies.

Main Results:

  • Detailed description of various assays used to evaluate enzymatic angiogenic functions.
  • Comparison of the strengths and weaknesses of different experimental approaches.
  • Guidance on selecting appropriate methods for studying synthetase-mediated angiogenesis.

Conclusions:

  • Specific assays are available to assess the angiogenic functions of enzymes like threonyl-tRNA synthetase.
  • Careful selection and application of these assays are necessary for accurate results.
  • Further research utilizing these methods will elucidate the role of synthetases in angiogenesis.
Keywords:
Aminoacyl-tRNA synthetaseAngiogenesis methodsCAM assayEndothelial cellsImageJMetaMorph

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