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E. coli Surface Properties Differ between Stream Water and Sediment Environments.

Xiao Liang1, Chunyu Liao2, Michael L Thompson3

  • 1Department of Agricultural and Biosystems Engineering, Iowa State University, Ames IA, USA.

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|November 17, 2016
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Summary

Environmental habitat influences Escherichia coli (E. coli) cell surface properties. Sediment-dwelling E. coli exhibit greater hydrophobicity and extracellular polymeric substance (EPS) content than water-dwelling strains, impacting their environmental fate.

Keywords:
E. coliparticlestreamsurface propertywater quality

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Area of Science:

  • Environmental Microbiology
  • Water Quality Assessment
  • Microbial Ecology

Background:

  • Escherichia coli (E. coli) is a key indicator organism in freshwater ecosystems.
  • Previous research has not fully explored the link between E. coli cell surface properties, genomic variations, and distinct environmental habitats.
  • Understanding these variations is crucial for accurate environmental fate and transport modeling.

Purpose of the Study:

  • To investigate variations in E. coli cell surface properties and genomic characteristics between stream water and stream sediment populations.
  • To determine if environmental habitat influences E. coli cell surface properties, considering genomic differences.
  • To assess the relationship between cell properties, EPS composition, and genomic similarity within different habitats.

Main Methods:

  • Collected 77 genomically distinct E. coli strains from stream water and sediments in the Upper Midwestern US.
  • Measured cell properties including hydrophobicity, zeta potential, net charge, total acidity, and EPS composition under controlled stream conditions (pH 8.0, 10 mM ionic strength, 22°C).
  • Analyzed genomic similarity using (GTG)5-PCR and correlated cell properties with habitat and genomic data.

Main Results:

  • Stream sediment E. coli showed significantly higher hydrophobicity, EPS protein, and EPS sugar content compared to water E. coli.
  • Sediment E. coli also exhibited less negative net charge and a higher point of zero charge.
  • A positive correlation between hydrophobicity and EPS protein was found in sediment strains, and habitat significantly influenced cell surface properties like hydrophobicity after accounting for genomic factors.

Conclusions:

  • Environmental habitat is a significant factor shaping E. coli cell surface properties, particularly hydrophobicity.
  • Distinct cell surface characteristics of sediment-associated E. coli may affect their interaction with environmental particles.
  • Incorporating cell property diversity is essential for robust environmental fate and transport models of E. coli.