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Updated: Mar 12, 2026

Chemical Triphosphorylation of Oligonucleotides
Published on: June 2, 2022
One-pot four-enzyme synthesis of thymidinediphosphate-l-rhamnose
Siqiang Li1, Hong Wang1, Juncai Ma1
1National Glycoengineering Research Center and Shandong Provincial Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University, 27 Shanda Nan Lu, Jinan 250100, China. guofenggu@sdu.edu.cn chenzg@sdu.edu.cn.
Abstract:
A new, robust one-pot four-enzyme synthetic method was developed for thymidinediphosphate-l-rhamnose starting from d-glucose-1-phosphate. The enzymes, Glc-1-P thymidylyltransferase, dTDP-Glc-4,6-dehydratase, dTDP-4-keto-6-deoxy-Glc-3,5-epimerase and dTDP-4-keto-Rha reductase were derived from Streptococcus pneumonia serotype 23F, expressed in Escherichia coli, and studied in detail to provide the first direct evidence for their functions.

