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Related Experiment Video

Updated: Mar 12, 2026

Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix

Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix

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Novel E2 Glycoprotein Tetramer Detects Hepatitis C Virus-Specific Memory B Cells.

Maude Boisvert¹, Wanrui Zhang², Elizabeth J Elrod²

¹Centre de Recherche du Centre Hospitalier de l'Université de Montréal, Montreal, Quebec H2X 0A9, Canada.

Journal of Immunology (Baltimore, Md. : 1950)

|November 17, 2016

Summary

Researchers developed a novel E2 glycoprotein tetramer to identify hepatitis C virus (HCV)-specific B cells. This tool enables better understanding of B cell responses and humoral immunity in HCV infection.

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Area of Science:

Immunology
Virology
Molecular Biology

Background:

Hepatitis C virus (HCV) infection often leads to persistent viral presence.
Antibodies targeting HCV envelope glycoproteins E1 and E2 are produced late in acute infection, but their role in viral clearance is debated.
Studying B cell responses in acute HCV infection is challenging due to difficulties in identifying and characterizing specific B cells.

Purpose of the Study:

To develop a novel tetramer tool for visualizing and characterizing hepatitis C virus (HCV)-specific B cells.
To investigate the presence and characteristics of E2 glycoprotein-specific B cells during acute and chronic HCV infection.
To analyze the B cell receptor (BCR) repertoire of HCV-specific B cells to understand clonal selection and affinity maturation.

Main Methods:

Development of a novel tetramer using the E2 glycoprotein ectodomain from the J6 strain (genotype 2a).
Longitudinal monitoring of E2-specific B cells in the peripheral blood of HCV-infected individuals using the tetramer.
Deep sequencing of the B cell receptor (BCR) from sorted E2-specific class-switched memory B cells.

Main Results:

E2 tetramer successfully visualized E2-specific B cells in peripheral blood of HCV-infected individuals.
HCV-specific class-switched memory B cells were detected in participants with late acute and chronic infections.
Deep sequencing revealed a focused BCR repertoire in E2-specific B cells, indicating clonal selection, expansion, and affinity maturation.

Conclusions:

The novel E2 tetramer is a valuable tool for specific and sensitive ex vivo characterization of rare HCV-specific B cells.
This methodology will enhance the understanding of humoral immune responses in HCV infection.
The findings provide insights into B cell dynamics and repertoire development during HCV infection.