Analysis of restriction endonuclease fragment patterns of DNA from Mycobacterium paratuberculosis

The DNA from 31 isolates and a reference strain of Mycobacterium paratuberculosis was digested individually with restriction endonucleases BstE II and Pst I. DNA fragments were separated by gel electrophoresis and analyzed. The isolates were from 23 American states, Argentina and Nova Scotia. Twenty-seven were isolated from cattle, two from goats and two from sheep. With the exception of one isolate from cattle, all had restriction endonuclease fragment patterns identical to the fragment patterns for the reference strain, M. paratuberculosis ATCC 19698T. These results confirm other reports and indicate that organisms identified as typical M. paratuberculosis isolates are genetically very similar. It may be possible to use restriction endonuclease analysis to differentiate isolates of M. paratuberculosis from other slowly growing mycobacteria. The genetic similarity also indicates that it may be possible to develop a diagnostic probe that is specific for M. paratuberculosis.