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Published on: May 1, 2018
A new experimental burn model with an infrared heater
Serbülent Güzey1, Ahmet Demirhan Dal2, İsmail Şahin3
1Department of Plastic Reconstructive and Aesthetic Surgery, Kasımpaşa Military Hospital, İstanbul-Turkey. drserbulent@yahoo.com.
Background:
This study was undertaken to develop new experimental burn injury model using conventional infrared heaters.
Methods:
21 Sprague-Dawley rats were divided into 3 groups. Portion of dorsal area was exposed to infrared radiation from distance of 50 cm to create burn injury. Length of exposure to heat for Group 1 was 5 minutes; Group 2 was exposed for 7½ minutes, and Group 3 was exposed for 10 minutes. Macroscopic and histopathological evaluations were utilized to demonstrate depth and characteristics of injury.
Results:
There was no burn injury in first group. Group 2 developed partial thickness burn, and result was full thickness burn injury in Group 3. In Groups 2 and 3 there was statistically significant difference in dermal collagen denaturation. Dermal injury depth was statistically significantly higher in Group 3 compared to Group 2.
Conclusion:
New experimental burn injury model is described using conventional infrared heaters. Standard variables pertaining to model were defined to produce burn injuries at predictable depth: 10 minutes of exposure from 50 cm distance for full thickness burn, and 7½ minutes of exposure from the same distance for partial thickness injury.
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