Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Attachment of Sister Chromatids02:57

Attachment of Sister Chromatids

4.1K
As cells progress into mitosis, the nuclear envelope breaks down, and the condensed chromosomes are exposed to the array of bipolar microtubules of the mitotic spindle. The kinetochore, a large, disc-shaped protein complex, is present at the centromere region of the sister chromatids and acts as a binding site for the microtubules.  Usually, the plus-end of a single microtubule is embedded within the kinetochore. However, some kinetochores first establish lateral contact with the side-wall...
4.1K
Attachment of Sister Chromatids02:57

Attachment of Sister Chromatids

1.8K
1.8K
Histone Variants at the Centromere02:30

Histone Variants at the Centromere

5.2K
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
5.2K
The Spindle Assembly Checkpoint02:19

The Spindle Assembly Checkpoint

3.9K
The spindle assembly checkpoint is a molecular surveillance mechanism ensuring the fidelity of chromosome segregation during anaphase. The checkpoint monitors the completion of all the prerequisite steps before chromosome segregation to determine whether the segregation process should proceed or be delayed.
Many proteins function together to control the spindle assembly checkpoint. Mutations affecting these proteins may allow cells to proceed into anaphase prematurely, resulting in the...
3.9K
Cohesins02:20

Cohesins

5.8K
Cohesin protein complexes are a molecular glue that holds two sister chromatids together. They play an important role both in mitosis and meiosis. In mitosis, all cohesin complexes present on the chromosomes are removed before the start of the anaphase stage.
Cohesin complexes in Meiotic Division
Meiosis involves two distinct rounds of chromosomal segregation and cell divisions— Meiosis I followed by Meiosis II – producing four daughter cells. Meiosis I includes the separation of...
5.8K
Cohesins02:20

Cohesins

2.4K
2.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

External Validation of the AS5F Score and the Role of Left Atrial Dilatation in Post-Stroke/TIA Atrial Fibrillation Detection.

Biomedicines·2026
Same author

Tobacco product use among middle and high school students in the United States: National Youth Tobacco Survey, 2025.

Nicotine & tobacco research : official journal of the Society for Research on Nicotine and Tobacco·2026
Same author

Structures of folding intermediates on BAM show diverse substrates fold by a conserved mechanism.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Mechanism of membrane perforation in rotavirus cell entry.

bioRxiv : the preprint server for biology·2026
Same author

Structures of folding intermediates on BAM show diverse substrates fold by a uniform mechanism.

bioRxiv : the preprint server for biology·2025
Same author

Time-varying predictors of e-cigarette and cigarette use trajectories from adolescence to emerging adulthood: a longitudinal analysis of US youth in the PATH Study, 2013-2020.

BMJ open·2025

Related Experiment Video

Updated: Mar 12, 2026

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
05:35

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

15.8K

Conserved Tetramer Junction in the Kinetochore Ndc80 Complex.

Roberto Valverde1, Jessica Ingram2, Stephen C Harrison1

  • 1Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, and Howard Hughes Medical Institute, 250 Longwood Avenue, Boston, MA 02115, USA.

Cell Reports
|November 17, 2016
PubMed
Summary

The Ndc80 complex forms a crucial rod-like structure connecting kinetochores to microtubules. Its crystal structure reveals conserved junctional interactions, suggesting binding sites for unknown cellular factors.

Keywords:
X-ray crystallographycell divisioncoiled-coil junctionkinetochore assemblykinetochore structure

More Related Videos

Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques
05:58

Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques

Published on: September 6, 2024

1.7K
Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

15.1K

Related Experiment Videos

Last Updated: Mar 12, 2026

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins
05:35

Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

Published on: March 3, 2016

15.8K
Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques
05:58

Author Spotlight: Efficient Nucleosome Reconstitution for Single-Molecule Techniques

Published on: September 6, 2024

1.7K
Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis
08:33

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

15.1K

Area of Science:

  • Cell Biology
  • Structural Biology
  • Molecular Motors

Background:

  • The Ndc80 complex is essential for kinetochore-microtubule attachment, a fundamental process in cell division.
  • It forms a long rod by joining two heterodimeric subcomplexes, spanning the gap between centromeres and spindle microtubules.
  • The heterotetrameric organization and junctional characteristics are evolutionarily conserved across species.

Purpose of the Study:

  • To determine the crystal structure of the Ndc80 complex, focusing on the conserved tetramer junction.
  • To elucidate the structural basis of the Ndc80 complex's rod-like architecture.
  • To identify potential binding sites for regulatory factors.

Main Methods:

  • X-ray crystallography was used to determine the structures of shortened Ndc80 complexes.
  • Structural analysis focused on the tetramer junction and globular ends of the complex.

Main Results:

  • Crystal structures of two "dwarf" Ndc80 complexes, containing the complete tetramer junction and globular ends, were determined.
  • The junction was found to connect two alpha-helical coiled coils via four-chain and three-chain overlap regions.
  • The structure revealed interactions among conserved amino acid residues at the junction.

Conclusions:

  • The conserved structure of the Ndc80 complex junction is critical for its function.
  • The identified conserved residues suggest a potential binding site for as-yet-unidentified cellular factors.
  • Further research is needed to identify these factors and their roles in kinetochore function.