MicroRNAs for osteosarcoma in the mouse: a meta-analysis
Junli Chang1,2, Min Yao1,2, Yimian Li1,2
1Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Abstract:
Osteosarcoma (OS) is the most common primary malignant bone carcinoma with high morbidity that happens mainly in children and young adults. As the key components of gene-regulatory networks, microRNAs (miRNAs) control many critical pathophysiological processes, including initiation and progression of cancers. The objective of this study is to summarize and evaluate the potential of miRNAs as targets for prevention and treatment of OS in mouse models, and to explore the methodological quality of current studies. We searched PubMed, Web of Science, Embase, Wan Fang Database, VIP Database, China Knowledge Resource Integrated Database, and Chinese BioMedical since their beginning date to 10 May 2016. Two reviewers separately screened the controlled studies, which estimate the effects of miRNAs on osteosarcoma in mice. A pair-wise analysis was performed. Thirty six studies with enough randomization were selected and included in the meta-analysis. We found that blocking oncogenic or restoring decreased miRNAs in cancer cells could significantly suppress the progression of OS in vivo, as assessed by tumor volume and tumor weight. This meta-analysis suggests that miRNAs are potential therapeutic targets for OS and correction of the altered expression of miRNAs significantly suppresses the progression of OS in mouse models, however, the overall methodological quality of studies included here was low, and more animal studies with the rigourous design must be carried out before a miRNA-based treatment could be translated from animal studies to clinical trials.
Insights
MicroRNAs (miRNAs) show promise for treating osteosarcoma (OS) by suppressing tumor growth in mouse models. However, current research quality is low, necessitating rigorous animal studies before clinical application.
Area of Science:
- Oncology
- Molecular Biology
- Gene Regulation
Background:
- Osteosarcoma (OS) is a prevalent and aggressive bone cancer primarily affecting children and young adults.
- MicroRNAs (miRNAs) are crucial gene regulators involved in cancer initiation and progression.
Purpose of the Study:
- To evaluate the therapeutic potential of miRNAs for osteosarcoma (OS) prevention and treatment in mouse models.
- To assess the methodological quality of existing miRNA-related osteosarcoma studies in animals.
Main Methods:
- A systematic literature search was conducted across multiple databases (PubMed, Web of Science, Embase, etc.) up to May 10, 2016.
- Thirty-six randomized controlled studies investigating miRNA effects on osteosarcoma in mice were included in a meta-analysis.
- Pair-wise analysis was performed to assess the impact of miRNA manipulation on tumor progression.
Main Results:
- Modulating miRNA expression—blocking oncogenic or restoring tumor-suppressive miRNAs—significantly inhibited osteosarcoma progression in vivo.
- Tumor volume and weight were significantly reduced following miRNA-based interventions in mouse models.
Conclusions:
- MicroRNAs represent promising therapeutic targets for osteosarcoma, with altered expression correction suppressing tumor growth in preclinical models.
- The overall methodological quality of included studies was low, highlighting the need for more rigorous animal research before clinical translation.
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