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Related Experiment Videos

A Mouse Model for Binge-Level Methamphetamine Use.

Shkelzen Shabani1, Sydney K Houlton1, Laura Hellmuth1

  • 1Department of Biology, Minot State University Minot, ND, USA.

Frontiers in Neuroscience
|November 18, 2016
PubMed
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Methamphetamine (MA) high drinking (MAHDR) mice exhibit binge-level MA intake, unlike low drinking (MALDR) mice, across varying concentrations and withdrawal periods. This genetic model is crucial for studying MA use disorders and developing treatments.

Area of Science:

  • Neuroscience
  • Pharmacology
  • Genetics

Background:

  • Binge and crash cycles of methamphetamine (MA) use are common in individuals with MA use disorders.
  • A validated genetic animal model for studying binge-level MA intake is currently lacking.

Purpose of the Study:

  • To determine if procedural variations can induce binge-level MA intake in selectively bred mouse lines.
  • To establish a genetic animal model for investigating MA use disorders.

Main Methods:

  • Utilized selectively bred MA high drinking (MAHDR) and low drinking (MALDR) mouse lines, along with progenitor strains (DBA/2J and F2 cross).
  • Examined the impact of MA concentration, bottle ratio (MA:water), and withdrawal period length on MA intake.
  • Employed two-bottle choice procedures with incrementally increasing MA concentrations and varying abstinence durations.
Keywords:
MAHDRMALDRabstinencegeneticselected lineself-administrationvoluntary consumptionwithdrawal

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Main Results:

  • MAHDR mice consumed significantly higher amounts of MA (up to 14.6 mg/kg) compared to MALDR mice (1.1 mg/kg) when MA concentration was increased.
  • A 3:1 MA:water bottle ratio escalated MA intake in MAHDR mice (29.1 mg/kg) and F2 mice (12.0 mg/kg), but not MALDR mice.
  • MAHDR mice maintained high MA intake irrespective of withdrawal period length (30-78 hours), while D2 mice reduced intake after 30-hour abstinence.

Conclusions:

  • MAHDR mice represent a robust genetic model for binge-level MA intake.
  • This model is suitable for studying the neurobiological effects of MA binge use.
  • The MAHDR model can facilitate the development and testing of pharmaceutical interventions for MA use disorders.