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Intracellular buffering.

R F Burton

    Respiration Physiology
    |April 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    Accurately measuring non-bicarbonate buffering (betaa) in tissues requires considering bicarbonate and other buffers. Factors like active pH regulation and cytoplasmic variations complicate betaa determination in intact tissues.

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    Area of Science:

    • Physiology
    • Biochemistry
    • Cell Biology

    Background:

    • Buffering is crucial for maintaining cellular pH homeostasis.
    • Bicarbonate and non-bicarbonate buffers play distinct roles in physiological pH regulation.
    • Accurate quantification of buffering capacity is essential for understanding cellular function.

    Purpose of the Study:

    • To discuss available estimates of the total non-bicarbonate buffer value (betaa) in intact tissues.
    • To analyze the contributions of proteins, dipeptides, and phosphates to cellular buffering.
    • To explore the implications of betaa for cellular function and extracellular fluid buffering.

    Main Methods:

    • Review and discussion of existing literature on betaa determination.
    • Analysis of factors complicating betaa measurement in intact tissues, including active pH regulation and cytoplasmic heterogeneity.

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  • Examination of potential errors in betaa estimates derived from homogenates due to in vivo pH heterogeneity.

    • Main Results:

    • The determination of betaa in intact tissues is complicated by active pH regulation and cytoplasmic heterogeneity.
    • Heterogeneity of pH in vivo can lead to errors when using homogenates for betaa estimation.
    • Individual contributions of proteins, dipeptides, and phosphates to betaa are discussed.

    Conclusions:

    • A high betaa is advantageous for cells with high glycolytic rates or those buffering extracellular fluid.
    • Non-protein buffer concentrations may be lower than osmolarity limits, potentially to avoid upsetting ionic gradients.
    • Understanding betaa is critical for comprehending cellular acid-base balance and function.