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Histone Purification from Saccharomyces cerevisiae.

Frederic Jourquin1, Vincent Géli2

  • 1Cancer Research Center of Marseille (CRCM), U1068 Inserm, UMR7258 CNRS, Aix Marseille University (AMU), Institut Paoli-Calmettes, Marseille, 13009, France.

Methods in Molecular Biology (Clifton, N.J.)
|November 18, 2016
PubMed
Summary

Researchers developed a robust method to purify histones from yeast. This technique enables quantitative analysis of specific posttranslational histone modifications, crucial for understanding nucleosome function.

Keywords:
Budding yeastChromatinHistonesPurification

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • Nucleosomes, the fundamental units of DNA packaging, are composed of histone proteins.
  • Histones are subject to various posttranslational modifications influencing nucleosome stability and function.
  • Understanding these modifications is key to deciphering gene regulation.

Purpose of the Study:

  • To establish a reliable protocol for purifying histones from Saccharomyces cerevisiae.
  • To enable quantitative analysis of posttranslational histone modifications.

Main Methods:

  • Development of a robust purification protocol for histones.
  • Utilizing yeast (Saccharomyces cerevisiae) as the model organism.

Main Results:

  • Successful and robust purification of histones achieved.
  • The protocol is suitable for quantitative analysis of specific histone modifications.

Conclusions:

  • The developed method provides a valuable tool for studying histone modifications.
  • This protocol facilitates deeper understanding of nucleosome dynamics and regulation.