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Active control of intracellular pH.

W F Boron

    Respiration Physiology
    |April 1, 1978
    PubMed
    Summary

    Squid giant axons actively remove excess acid using an energy-dependent process. This mechanism involves exchanging external bicarbonate for internal chloride ions, consuming ATP.

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    Area of Science:

    • Neurobiology
    • Cell Physiology
    • Biochemistry

    Background:

    • The squid giant axon is a model system for studying neuronal function.
    • Cellular acid extrusion is crucial for maintaining intracellular pH homeostasis.
    • Energy-dependent ion transport mechanisms are vital for cell survival.

    Purpose of the Study:

    • To investigate the mechanism of acid extrusion in the squid giant axon.
    • To identify the ions and energy source involved in this process.

    Main Methods:

    • Loading the squid giant axon interior with acid.
    • Observing the cell's response to internal acidification.
    • Analyzing ion exchange and energy consumption during acid extrusion.

    Main Results:

    • The squid giant axon actively extrudes excess internal acid.
    • This acid extrusion is an energy-requiring process.
    • The mechanism involves the exchange of external bicarbonate (HCO3-) for internal chloride (Cl-) ions.
    • Adenosine triphosphate (ATP) is consumed during this process.

    Conclusions:

    • The squid giant axon employs a specific ion transport system for acid extrusion.
    • This system utilizes external bicarbonate and internal chloride, coupled with ATP hydrolysis.
    • Understanding this mechanism provides insights into cellular pH regulation in neurons.

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