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Anti-high endothelial venule monoclonal antibody HECA-452 recognizes plasmacytoid T cells and delineates an
F Facchetti1, C de Wolf-Peeters, J J van den Oord
1Department of Pathology, Catholic University of Leuven, Belgium.
So-called plasmacytoid T cells represent a subset of monocyte related cells, which share with endothelium the CD36+ CD11b- (OKM5+ OKM1-) phenotype. The reactivity of plasmacytoid T cells with rat monoclonal antibody HECA-452, highly specific for high endothelial venules, was analyzed in reactive lymph nodes. In all cases, HECA-452 not only labelled the endothelium of high endothelial venules, but also strongly reacted with singular and clustered plasmacytoid T cells. The HECA-452 positivity for high endothelial venules and plasmacytoid T cells visualized a lymph node compartment extending from the subcapsular sinus to the corticomedullary junction. This compartment surrounded the composite nodule and was designated the "extranodular" compartment. The co-occurrence of plasmacytoid T cells and high endothelial venules in this extranodular compartment, together with their immunophenotypical similarities, may be indicative of functional co-operations.
So-called plasmacytoid T cells represent a subset of monocyte related cells, which share with endothelium the CD36+ CD11b- (OKM5+ OKM1-) phenotype. The reactivity of plasmacytoid T cells with rat monoclonal antibody HECA-452, highly specific for high endothelial venules, was analyzed in reactive lymph nodes. In all cases, HECA-452 not only labelled the endothelium of high endothelial venules, but also strongly reacted with singular and clustered plasmacytoid T cells. The HECA-452 positivity for high endothelial venules and plasmacytoid T cells visualized a lymph node compartment extending from the subcapsular sinus to the corticomedullary junction. This compartment surrounded the composite nodule and was designated the "extranodular" compartment. The co-occurrence of plasmacytoid T cells and high endothelial venules in this extranodular compartment, together with their immunophenotypical similarities, may be indicative of functional co-operations.