Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Phase Contrast and Differential Interference Contrast Microscopy

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Safety and efficacy of decompressive craniectomy versus standard craniotomy for large acute epidural haematoma with tentorial herniation in China (PREDICT-AEDH): a nationwide, multicentre, open-label, parallel-group, randomised controlled trial.

The Lancet. Neurology·2026

Same author

Is the Effect of Intensive Antihypertensive Treatment in Acute Intracerebral Hemorrhage Dependent on Hematoma Volume? A Traditional Meta-analysis of the Effect of Antihypertensive Regimens, a Bayesian Network Meta-analysis of the Mortality of Antihypertensive Drugs and Systematic Review.

CNS drugs·2025

Same author

Accurate neuron segmentation method for one-photon calcium imaging videos combining convolutional neural networks and clustering.

Communications biology·2024

Same author

The recent two decades of traumatic brain injury: a bibliometric analysis and systematic review.

International journal of surgery (London, England)·2024

Same author

Which is the best treatment for melanoma brain metastases? A Bayesian network meta-analysis and systematic review.

Critical reviews in oncology/hematology·2024

Same author

[Retracted] Deoxypodophyllotoxin inhibits cell viability and invasion by blocking the PI3K/Akt signaling pathway in human glioblastoma cells.

Oncology reports·2023

Search research articles

Related Experiment Video

Updated: Mar 12, 2026

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

Quantitative phase imaging method based on an analytical nonparaxial partially coherent phase optical transfer

Yijun Bao, Thomas K Gaylord

Journal of the Optical Society of America. A, Optics, Image Science, and Vision

|November 19, 2016

Summary

This summary is machine-generated.

A new nonparaxial multifilter phase imaging with partially coherent light (MFPI-PC) method overcomes paraxial limitations. This advanced technique enhances imaging accuracy without increasing computational cost, offering a more realistic approach to phase imaging.

More Related Videos

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Published on: February 12, 2014

Related Experiment Videos

Last Updated: Mar 12, 2026

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

Published on: April 7, 2014

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Shaping the Amplitude and Phase of Laser Beams by Using a Phase-only Spatial Light Modulator

Published on: January 28, 2019

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Time Multiplexing Super Resolving Technique for Imaging from a Moving Platform

Published on: February 12, 2014

Area of Science:

Optics and Photonics
Image Processing
Microscopy

Background:

Multifilter phase imaging with partially coherent light (MFPI-PC) is a quantitative phase imaging technique.
Current MFPI-PC methods rely on the paraxial approximation, limiting their applicability to non-ideal imaging scenarios.
There is a need for MFPI-PC methods that can accurately image objects under nonparaxial conditions.

Purpose of the Study:

To develop and validate an analytical nonparaxial partially coherent phase optical transfer function.
To extend the MFPI-PC method to handle realistic nonparaxial imaging conditions.
To improve the accuracy and remove systematic errors in MFPI-PC.

Main Methods:

Derivation of an analytical nonparaxial partially coherent phase optical transfer function.
Implementation of obliquity factor correction for nonparaxial imaging.
Validation through numerical simulations on diverse phase objects and experimental measurements on a microlens array.

Main Results:

The derived nonparaxial MFPI-PC method demonstrates significantly higher imaging accuracy compared to the paraxial approach.
The nonparaxial MFPI-PC with obliquity factor correction eliminates systematic errors observed in the paraxial version.
The analytical nature of the nonparaxial method results in a negligible increase in computation time.

Conclusions:

The developed nonparaxial MFPI-PC method provides a more accurate and robust solution for quantitative phase imaging.
This advancement enables the application of MFPI-PC to a wider range of realistic, nonparaxial imaging scenarios.
The method offers improved performance without a significant computational penalty, making it practical for various applications.