Preparation of EGF labeled liposomes and their uptake by hepatocytes

The preparation of epidermal growth factor (EGF) bearing liposomes (EGF-liposomes) and their uptake by rat hepatocytes were described. EGF was bound to the liposomal surface by the disulfide bridge linkage using a heterobifunctional cross-linking reagent, N-hydroxysuccinimidyl-3-(2-pyridyl-dithio) propionate. EGF-liposomes were taken up in a significant amount by rat hepatocytes in an EGF receptor mediated manner, and their uptake was dependent on the amount of labeled EGF coupled to the liposomal surface. These results raise the possibility that the EGF-liposomes may be an effective drug carrier to the cells having EGF-receptors like some cancer cells.