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Comet Assay as an Indirect Measure of Systemic Oxidative Stress
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Coffee and oxidative stress: a human intervention study.

Sergey Shaposhnikov1,2, Thomas Hatzold3,4, Naouale El Yamani5

  • 1NorGenoTech AS, Totenvegen 2049, 2848, Skreia, Norway. sas@norgenotech.no.

European Journal of Nutrition
|November 19, 2016
PubMed
Summary

This study found that drinking up to 5 cups of coffee daily had no significant antioxidant effects or impact on the health markers of healthy volunteers. Coffee consumption did not alter DNA or lipid oxidation, blood pressure, or key clinical markers.

Keywords:
CoffeeDNA oxidationHuman intervention trialLipid oxidation

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Area of Science:

  • Nutritional Science
  • Biochemistry
  • Human Physiology

Background:

  • Coffee contains phytochemicals with known antioxidant properties.
  • Previous research suggests potential health benefits of coffee consumption.
  • The specific impact of regular coffee intake on oxidative stress markers in humans requires further investigation.

Purpose of the Study:

  • To evaluate the antioxidant potential of coffee in healthy human volunteers.
  • To assess the effects of moderate coffee consumption on biomarkers of oxidative damage.
  • To determine if coffee intake influences key clinical chemistry and lipid profiles.

Main Methods:

  • A randomized, placebo-controlled trial involving 160 healthy subjects over 8 weeks.
  • Participants consumed 3 or 5 cups of study coffee or water daily.
  • Analysis included serum lipids, clinical chemistry, DNA damage (comet assay), urinary isoprostanes, and liver enzymes.

Main Results:

  • No significant changes were observed in markers of DNA and lipid oxidation.
  • A slight increase in serum creatinine and a significant elevation in gamma-glutamyl transaminase (in the 5 cups/day group) were noted.
  • Blood lipids, glucose, insulin, and inflammatory markers remained unchanged; blood pressure was unaffected.

Conclusions:

  • Up to 5 cups of coffee per day demonstrated no detectable beneficial or harmful effects on the health of healthy subjects in this trial.
  • The study suggests that moderate coffee consumption does not significantly impact oxidative stress markers or general health parameters in this population.
  • Further research may explore different dosages or populations to fully elucidate coffee's health effects.