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Updated: Mar 11, 2026
06:14
Separation and Fractionation of Cell Wall and Cell Membrane Proteins from Mycobacterium tuberculosis for Downstream Protein Analysis
Published on: September 26, 2025
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Genome scale identification, structural analysis, and classification of periplasmic binding proteins from
Padmani Sandhu1, Monika Kumari1, Kamal Naini1
1Centre for Computational Biology and Bioinformatics, School of Life Sciences, Central University of Himachal Pradesh, Shahpur, District Kangra, Himachal Pradesh, 176206, India.
Current Genetics
|November 19, 2016
Summary
Researchers identified 37 periplasmic-binding proteins (PBPs) in Mycobacterium tuberculosis, including 11 novel ones. This expands our understanding of Mtb
Area of Science:
- Microbiology
- Molecular Biology
- Biochemistry
Background:
- Periplasmic-binding proteins (PBPs) are crucial for solute transport and signaling in bacteria.
- Limited data exists on periplasmic proteins in Mycobacterium tuberculosis (Mtb).
Purpose of the Study:
- To perform a genome-wide identification and functional annotation of Mtb periplasmic-binding proteins.
- To expand the known repertoire of Mtb PBPs and understand their roles.
Main Methods:
- Bioinformatic analysis of the Mtb proteome.
- Identification based on signature characteristics and functional motifs of PBPs.
Main Results:
- Identified 37 putative periplasmic-binding proteins in Mtb.
- Categorized these proteins based on their association with membrane transport and signaling pathways.
- Discovered 11 novel proteins previously unannotated as PBPs in Mtb.
Conclusions:
- This study provides a comprehensive overview of the Mtb periplasmic binding proteome.
- These proteins are potentially involved in critical patho-physiological functions.
- Identified PBPs represent potential novel drug targets for tuberculosis treatment.
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