Aquamicrobium soli sp. nov., a bacterium isolated from a chlorobenzoate-contaminated soil

An aerobic, Gram-stain negative, short rod-shaped, asporogenous, non-motile bacterium designated strain NK8^T was isolated from a chlorobenzoate contaminated soil in China. Strain NK8^T was observed to grow optimally at pH 7.0, 30 °C and in the absence of NaCl in LB medium. The G + C content of the total DNA of strain NK8^T was found to be 65.5 mol%. The 16S rRNA gene sequence of strain NK8^T showed high similarity to that of Aquamicrobium aerolatum Sa14^T (97.3%), followed by Aquamicrobium lusatiense S1^T (96.7%) and Mesorhizobium sangali SCAU7^T (96.6%). The DNA-DNA relatedness between strain NK8^T and A. aerolatum Sa14^T was 35.5 ± 0.9%. The major fatty acids of strain NK8^T were determined to be C_19:0 cyclo ω8c (45.6%), C_18:1 ω7c (33.4%) and C_16:0 (8.4%). The respiratory quinone was found to be ubiquinone Q-10. The major polyamine was found to be spermidine. The polar lipid profile include the major compounds phosphatidylcholine and diphosphatidylglycerol, and moderate amounts of phosphatidylethanolamine, phosphatidylmonomethylethanolamine, aminolipid and phospholipid. Based on the differential biochemical and physiological characteristics, the geno-, chemo- and phenotypic characteristics, strain NK8^T is proposed to represent a novel species of the genus Aquamicrobium, Aquamicrobium soli sp. nov. The type strain is NK8^T (=KCTC 52165^T=CCTCC AB2016045^T).