Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pharmacological Inhibition of FKBP51 Mitigates Early Life Adversity-Induced Social Deficits in Male Mice.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

Disruption of the developmental factor Otp in the adult male forebrain reveals its diverse physiological functions.

Endocrinology·2026
Same author

Intensity and exposure proximity as determinants of differential stress-related health outcomes.

Molecular psychiatry·2026
Same author

Mineralocorticoid Receptor in Glutamatergic Neurons Modulates Anxiety Exclusively in Male Mice Via Regulation of the Actin-Bundling Factor Fam107a.

Biological psychiatry global open science·2026
Same author

Single-cell characterization of the adult male hippocampus suggests a prominent, and cell-type specific, role for Nrgn and Sgk1 in response to a social stressor.

Molecular psychiatry·2026
Same author

Folliculostellate Cells at the Crossroads of Inflammation, Endocrine Regulation, and Aging in the Pituitary Gland.

Neuroimmunomodulation·2025

Related Experiment Video

Updated: Mar 11, 2026

Author Spotlight: Insights and Innovations in Gene Expression Manipulation Techniques for Choroid Plexus Research
04:43

Author Spotlight: Insights and Innovations in Gene Expression Manipulation Techniques for Choroid Plexus Research

Published on: June 16, 2023

1.6K

Genetically dissecting P2rx7 expression within the central nervous system using conditional humanized mice.

Michael W Metzger1, Sandra M Walser1, Fernando Aprile-Garcia2,3

  • 1Max Planck Institute of Psychiatry, 80804, Munich, Germany.

Purinergic Signalling
|November 19, 2016
PubMed
Summary
This summary is machine-generated.

A new humanized mouse model reveals the central nervous system distribution of the P2X7 receptor (P2X7R). This tool aids in understanding P2X7R

Keywords:
Gene expressionKnockoutMouse modelP2X7 receptorP2rx7 genePore formation

More Related Videos

A Non-random Mouse Model for Pharmacological Reactivation of Mecp2 on the Inactive X Chromosome
08:27

A Non-random Mouse Model for Pharmacological Reactivation of Mecp2 on the Inactive X Chromosome

Published on: May 22, 2019

6.9K
Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells
11:47

Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells

Published on: April 3, 2019

9.9K

Related Experiment Videos

Last Updated: Mar 11, 2026

Author Spotlight: Insights and Innovations in Gene Expression Manipulation Techniques for Choroid Plexus Research
04:43

Author Spotlight: Insights and Innovations in Gene Expression Manipulation Techniques for Choroid Plexus Research

Published on: June 16, 2023

1.6K
A Non-random Mouse Model for Pharmacological Reactivation of Mecp2 on the Inactive X Chromosome
08:27

A Non-random Mouse Model for Pharmacological Reactivation of Mecp2 on the Inactive X Chromosome

Published on: May 22, 2019

6.9K
Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells
11:47

Real-time Live-cell Flow Cytometry to Investigate Calcium Influx, Pore Formation, and Phagocytosis by P2X7 Receptors in Adult Neural Progenitor Cells

Published on: April 3, 2019

9.9K

Area of Science:

  • Neuroscience
  • Pharmacology
  • Genetics

Background:

  • The purinergic P2X7 receptor (P2X7R) is implicated in central nervous system (CNS) disorders.
  • Accurate P2X7R distribution and localization data are crucial for understanding its role in brain disease.
  • Existing tools have limitations in resolving P2X7R expression patterns.

Purpose of the Study:

  • To develop a humanized mouse model for studying the human P2X7R in vivo.
  • To characterize the functionality and species-specific properties of the humanized P2X7R.
  • To precisely map the expression of P2X7R in the mouse brain.

Main Methods:

  • Generation of a genetically humanized mouse model for the P2X7R.
  • Assessment of humanized P2X7R functionality and response to agonists/antagonists (BzATP, TFP).
  • Utilized Cre-lox system for spatial and temporal control of human P2rx7 allele inactivation.

Main Results:

  • The humanized P2X7R exhibits species-specific activation and modulation characteristics.
  • The conditional null allele effectively inactivates all P2rx7 splice variants.
  • P2X7R is confirmed in hippocampal glutamatergic pyramidal neurons, astrocytes, oligodendrocytes, and microglia.

Conclusions:

  • The humanized mouse model enables detailed in vivo assessment of human P2X7R function.
  • This model facilitates the evaluation of P2X7R-targeting drugs for CNS pathologies.
  • The conditional allele supports future loss-of-function studies in relevant CNS disease models.