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Related Experiment Video

Updated: Jan 8, 2026

The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach
10:00

The Zebrafish Tol2 System: A Modular and Flexible Gateway-Based Transgenesis Approach

Published on: November 30, 2022

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Transgenic Zebrafish.

Xiaowen Chen1, Dafne Gays2, Massimo M Santoro3,4

  • 1Vesalius Research Center, VIB-KUL, O&N 9, 9e verd, Campus Gasthuisberg, Herestraat 49, bus 912, 3000, Leuven, Belgium.

Methods in Molecular Biology (Clifton, N.J.)
|November 19, 2016
PubMed
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Zebrafish transgenesis using Tol2 technology allows in vivo visualization of vascular tissues and endothelial cells. This method aids functional studies of tumor angiogenesis in vertebrate embryos.

Area of Science:

  • * Molecular Biology
  • * Developmental Biology
  • * Genetics

Background:

  • * Transgenic animals are crucial tools in modern research, enabling the study of gene function.
  • * Zebrafish are increasingly utilized for transgenesis due to their accessible embryos and rapid development.
  • * Combining zebrafish transgenesis with in vivo imaging offers powerful insights into vertebrate embryonic development.

Purpose of the Study:

  • * To describe the Tol2-based methodology for generating transgenic zebrafish.
  • * To highlight applications in visualizing vascular tissues and endothelial cells in vivo.
  • * To demonstrate the utility for functional tumor angiogenesis studies.

Main Methods:

  • * Generation of transgenic zebrafish using the Tol2 transposon system.
Keywords:
Gateway cloning systemTol2 systemTransgenesisZebrafish embryos

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  • * In vivo imaging techniques for observing embryonic vasculature.
  • * Application of transgenic lines for studying tumor angiogenesis.

    • Main Results:

    • * Successful generation of transgenic zebrafish lines using Tol2 methodology.
    • * Effective in vivo visualization of vascular development and endothelial cells.
    • * Demonstrated utility in functional studies of tumor angiogenesis.

    Conclusions:

    • * The Tol2-based method is a robust technique for creating transgenic zebrafish.
    • * Transgenic zebrafish provide a valuable model for in vivo vascular studies.
    • * This approach facilitates research into tumor angiogenesis and related vascular processes.