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Related Experiment Video

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The Rip1Tag2 Transgenic Mouse Model.

Ruben Bill1, Gerhard Christofori2

  • 1Department of Biomedicine, University of Basel, Mattenstrasse 28, 4058, Basel, Switzerland.

Methods in Molecular Biology (Clifton, N.J.)
|November 19, 2016
PubMed
Summary
This summary is machine-generated.

This study details methods for analyzing tumor blood and lymphatic vessels in Rip1Tag2 mice. These techniques assess vessel morphology, function, and tumor cell proliferation for cancer research.

Keywords:
Anti-angiogenic therapyEndothelial cellsImmunofluorescenceInsulinomaPNETRip1Tag2Tumor angiogenesis

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Area of Science:

  • Oncology
  • Vascular Biology
  • Immunohistochemistry

Background:

  • The Rip1Tag2 mouse model is crucial for studying beta-cell carcinogenesis, tumor angiogenesis, and anti-angiogenic therapy responses.
  • Assessing blood and lymphatic vessel phenotypes and functionality is essential for understanding tumor development and treatment efficacy.

Purpose of the Study:

  • To describe protocols for evaluating tumor vascular and lymphatic characteristics in the Rip1Tag2 mouse model.
  • To provide methods for assessing tumor cell proliferation and apoptosis.

Main Methods:

  • Immunofluorescence microscopy is employed to analyze key parameters.
  • Protocols cover tumor blood vessel morphology (pericyte coverage), functionality (perfusion, leakiness, hypoxia), and lymphatic tumor coverage.
  • Methods also include assessment of tumor cell proliferation and apoptosis.

Main Results:

  • The chapter provides detailed protocols for comprehensive vascular and cellular analysis.
  • These methods enable in-depth characterization of the tumor microenvironment.

Conclusions:

  • The described immunofluorescence-based protocols are vital for dissecting tumor angiogenesis and response to therapy in the Rip1Tag2 model.
  • These analyses are fundamental for advancing research in beta-cell carcinogenesis and anti-angiogenic treatments.