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Zika Virus: Quantification, Propagation, Detection, and Storage.

Darrell S Agbulos1, Larissa Barelli1,2, Bryan V Giordano1

  • 1Centre for Biotechnology, Brock University, St. Catharines, Ontario, Canada.

Current Protocols in Microbiology
|November 19, 2016
PubMed
Summary

This study details laboratory methods for Zika virus (ZIKV) quantification, propagation, and storage. It also outlines safety protocols for handling this Biosafety Level 2 pathogen.

Keywords:
Vero cellsZika virusdetectioninfectionplaque assay

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Area of Science:

  • Virology
  • Microbiology
  • Public Health

Background:

  • Zika virus (ZIKV), a Flaviviridae family member, emerged in Uganda in 1947.
  • Recent South American outbreaks linked ZIKV to microcephaly and Guillain-Barré syndrome.
  • Standardized research protocols are crucial due to increased ZIKV interest.

Purpose of the Study:

  • To describe essential laboratory techniques for ZIKV research.
  • To outline safe handling and storage procedures for ZIKV.
  • To provide guidance for researchers working with ZIKV.

Main Methods:

  • Laboratory techniques for ZIKV quantification.
  • Methods for ZIKV propagation and cell culture.
  • Procedures for ZIKV storage and preservation.

Main Results:

  • Established protocols for ZIKV quantification.
  • Detailed methods for ZIKV propagation.
  • Recommended storage conditions for ZIKV.
  • Reviewed safety protocols for handling ZIKV.

Conclusions:

  • Standardized laboratory protocols are essential for ZIKV research.
  • Proper handling and storage are critical for ZIKV research integrity.
  • Adherence to Biosafety Level 2 guidelines is necessary for ZIKV safety.