Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Feedback Regulation of Calcium Concentration

Feedback Regulation of Calcium Concentration

Calcium is an essential signaling molecule required for various cellular functions. Calcium pumps and ion channels on cell and organellar membranes, such as those on the endoplasmic reticulum (ER), regulate calcium concentrations inside the cell. They remain closed, keeping the cytosolic calcium levels low at a resting state.
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...

Calmodulin-dependent Signaling

Calmodulin-dependent Signaling

Calmodulin (CaM) is a calcium-binding protein in eukaryotes that controls various calcium-regulated cellular processes. It has four calcium-binding sites that bind calcium to form the calcium-calmodulin ( Ca2+-CaM) complex. GPCR stimulation increases the calcium levels in the cells that bind to CaM and induces a conformational change.
The Ca2+-CaM complex does not have enzymatic activity by itself. Instead, the complex binds downstream target proteins, including membrane proteins or enzymes,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Interplay between ADP-Ribosylation and Androgen Receptor Function in Prostate Cancer.

Current pharmaceutical design·2026

Same author

Evolving Treatments and Resistance Mechanisms in Prostate Cancer Therapeutics.

ACS pharmacology & translational science·2026

Same author

Electrochemical Nitrate Reduction through Cooperative Redox Participation of the CoMo<sub><i>x</i></sub> Cluster-Anchored γ-FeO(OH) Nanocore.

Inorganic chemistry·2026

Same author

Ayurvedic Phytochemicals in Oncology: ADP-Ribosylation as a Molecular Nexus.

Cells·2025

Same author

Heterogenization of Cobalt-Phosphotungstate with Triazolium-Polymer through Ion Pairing for Facile Nitrate Adsorption and Electroreduction to Ammonia.

ChemSusChem·2025

Same author

Deciphering the Role of Functional Ion Channels in Cancer Stem Cells (CSCs) and Their Therapeutic Implications.

International journal of molecular sciences·2025

Search research articles

Related Experiment Video

Updated: Mar 11, 2026

Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca2+ Signaling In Situ

Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca2+ Signaling In Situ

Published on: January 7, 2019

Spatial Ca²⁺ profiling: decrypting the universal cytosolic Ca²⁺ oscillation.

Krishna Samanta¹, Anant B Parekh¹

¹Department of Physiology, Anatomy and Genetics, University of Oxford, Parks Road, Oxford, OX1 3PT, UK.

The Journal of Physiology

|November 19, 2016

Summary

Calcium (Ca2+) oscillations, triggered by cell surface receptors, encode signals not just in spike timing but also in spatial patterns. This spatial signaling near membrane channels enhances cellular communication.

Keywords:

calcium channel cytosolic calcium mitochondria

More Related Videos

Imaging Local Ca2+ Signals in Cultured Mammalian Cells

Imaging Local Ca2+ Signals in Cultured Mammalian Cells

Published on: March 3, 2015

Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators

Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators

Published on: March 22, 2019

Related Experiment Videos

Last Updated: Mar 11, 2026

Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca2+ Signaling In Situ

Applications of Spatio-temporal Mapping and Particle Analysis Techniques to Quantify Intracellular Ca2+ Signaling In Situ

Published on: January 7, 2019

Imaging Local Ca2+ Signals in Cultured Mammalian Cells

Imaging Local Ca2+ Signals in Cultured Mammalian Cells

Published on: March 3, 2015

Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators

Dissection of Local Ca2+ Signals in Cultured Cells by Membrane-targeted Ca2+ Indicators

Published on: March 22, 2019

Area of Science:

Cellular biology
Biochemistry
Signal transduction

Background:

Cell surface receptor stimulation triggers phospholipase C, producing inositol trisphosphate.
Inositol trisphosphate generates oscillations in cytosolic calcium (Ca2+), a key second messenger.
Calcium signaling information is encoded in amplitude and frequency of Ca2+ spikes.

Purpose of the Study:

To investigate the role of spatial profiles in calcium (Ca2+) oscillations.
To explore how spatial patterns of Ca2+ signaling contribute to information transduction.
To understand the significance of Ca2+ microdomains in cellular communication.

Main Methods:

Stimulation of cell-surface receptors coupled to phospholipase C.
Monitoring cytosolic Ca2+ oscillations.
Analysis of spatial patterns of Ca2+ signaling.

Main Results:

Calcium (Ca2+) oscillations exhibit spatial profiles that encode signaling information.
Distinct Ca2+ microdomains near CRAC and IP3-gated channels signal to specific downstream targets.
Spatial profiling enhances the transduction power of Ca2+ oscillations.

Conclusions:

The spatial dimension of calcium (Ca2+) oscillations is a critical component of cellular signaling.
Spatial Ca2+ microdomains provide targeted signaling to distinct downstream effectors.
Understanding spatial Ca2+ dynamics deepens our knowledge of cellular communication.