Related Experiment Video For 3D AGGREGATES
Updated: Mar 11, 2026
Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
Published on: April 12, 2015
Protein Expression Differences of 2-Dimensional and Progressive 3-Dimensional Cell Cultures of
Maddaly Ravi1, Divya K Mohan1, Bellona Sahu1
1Faculty of Biomedical Sciences, Department of Human Genetics, Sri Ramachandra University, Porur, Chennai, Tamil Nadu, 600116, India.
Abstract:
Non-small-cell-lung-cancer (NSCLC) constitutes about 75-80% of lung cancers. The challenge to tackle cancers is in early diagnosis and arriving at safer therapeutic options. In vitro studies using cancer cell lines continue to contribute significantly in understanding cancers. Cell culture methods have evolved and the recent developments in 3 dimensional (3D) cell cultures are inducing greater resemblance of the in vitro cultured cells with in vivo conditions. In this study, we established 3D aggregates of H460 cell line on agarose hydrogels and studied the protein expression differences among cells grown as monolayers (2D) and the progressively developing 3D aggregates from days 2 to 10. Analysis included matching of those proteins expressed by the developing aggregates and the available literature on progressing tumors in vivo. J. Cell. Biochem. 118: 1648-1652, 2017. © 2016 Wiley Periodicals, Inc.
