Single-Layer Ternary Chalcogenide Nanosheet as a Fluorescence-Based "Capture-Release" Biomolecular Nanosensor

Area of Science:

• Materials Science
• Nanotechnology
• Biomedical Engineering

Background:

• Two-dimensional (2D) single-layer ternary chalcogenide nanosheets offer unique properties for biosensing applications.
• Developing sensitive and specific biomolecular nanosensors is crucial for early disease detection.

Purpose of the Study:

• To demonstrate the novel application of 2D ternary chalcogenide nanosheets as
• capture-release
• fluorescence-based biomolecular nanosensors.
• To develop a highly sensitive and specific nanosensor for detecting Plasmodium lactate dehydrogenase, a malarial biomarker.

Main Methods:

• Utilized ultrathin Ta₂ NiS₅ nanosheets as a fluorescence quencher.
• Employed a nucleic acid probe for capturing and releasing the target biomolecule.
• Investigated the nanosensor's performance in homogeneous and heterogeneous solutions, including serum.

Main Results:

• The Ta₂ NiS₅ nanosheet-based nanosensor exhibited high sensitivity and specificity for the target biomolecule.
• The
• capture-release
• mechanism demonstrated effective detection and differentiation of the target in complex biological samples.
• The nanosensor showed exceptional specificity in both homogeneous and heterogeneous solutions.

Conclusions:

• The developed ternary chalcogenide nanosheet-based nanosensor is a promising tool for rapid and accurate biomolecular detection.
• The simple
• capture-release
• method facilitates the development of portable and rapid biosensors.
• This approach holds potential for detecting a wide range of chemical and biological species.