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Updated: Mar 11, 2026
In Vivo Augmentation of Gut-Homing Regulatory T Cell Induction
Published on: January 22, 2020
Forkhead box protein-3 (Foxp3)-producing dendritic cells suppress allergic response
X-Y Liu1, L-Z Xu2,3, X-Q Luo2,3
1MOE Key Laboratory of Aquatic Product Safety, School of Life Sciences, School of Marine Sciences, Sun Yat-Sen University, Guangzhou, 510275, China.
Background:
The generation of the tolerogenic dendritic cells (DC) is not fully understood yet. Forkhead box protein-3 (Foxp3) is an important molecule in the immune tolerance. This study tests a hypothesis that DCs express Foxp3, which can be upregulated by Staphylococcal enterotoxin B (SEB).
Methods:
The expression of Foxp3 by DCs was evaluated by real-time RT-PCR, Western blotting, flow cytometry, and chromatin immunoprecipitation assay.
Results:
We observed that mice treated with SEB at 0.25-0.5 μg/mouse showed high frequencies of transforming growth factor (TGF)-β-producing CD4+ T cells and TGF-β-producing DCs in the intestine, while the IL-4+ CD4+ T cells and TIM4+ DCs were dominated in the intestine in mice treated with SEB at 1-10 μg/mouse. Treating DCs with SEB in the culture induced high levels of Foxp3 at the TGF-β promoter locus. The function of Foxp3 was blocked by STAT6 (signal transducer and activator transcription-6); the latter was induced by exposing DCs to SEB in the culture at doses of 100-400 ng/ml. Treating allergic mice with specific immunotherapy (SIT) together with SEB significantly promoted the therapeutic effects on the allergic responses than treating with SIT alone.
Conclusion:
Dendritic cells have the capacity to express Foxp3, which can be upregulated by exposure to SEB.
