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Establishment of complete blood count reference intervals for Chinese preschoolers
Guo-Cheng Wang1, Na Li1, Cui Niu2
1Department of Clinical Laboratory, Shunyi Maternal and Children's Hospital of Beijing Children's Hospital, Shunyi, Beijing, China.
Insights
This study established local complete blood count reference intervals for healthy Chinese preschoolers. These region-specific ranges are crucial for accurate clinical laboratory test interpretation in this age group.
Area of Science:
- Clinical Laboratory Science
- Pediatric Hematology
- Reference Interval Determination
Background:
- Accurate interpretation of clinical laboratory test results relies on established reference intervals.
- Previous studies have not adequately defined reference intervals for complete blood counts in Chinese preschoolers.
Purpose of the Study:
- To establish local, age-specific reference intervals for complete blood count parameters in healthy Chinese preschoolers.
- To provide a basis for accurate hematological assessment in this demographic.
Main Methods:
- Analysis of 3,821 blood specimens from children aged 4 months to 6 years.
- Determination of complete blood counts using a Sysmex XT-4000i Automated Hematology Analyzer.
- Calculation of nonparametric 2.5th to 97.5th percentile reference ranges following CLSI EP28-A3c guidelines.
Main Results:
- Established reference intervals for total white blood cell count, red blood cell count, hemoglobin, hematocrit, and various red blood cell indices (MCH, MCHC, MCV, RDW-SD, RDW-CV).
- Determined reference intervals for platelet count and related parameters (PCT, MPV, PDW, P-LCR).
- Provided distinct reference intervals for male and female preschoolers for each parameter.
Conclusions:
- Successfully established local complete blood count reference intervals for apparently healthy preschoolers in China.
- Highlights the necessity of developing region-specific reference intervals for accurate pediatric hematological evaluation.
Background:
Reference intervals are critical for interpreting test results of a clinical laboratory. The aim of this study was to establish local reference intervals of complete blood count for healthy preschoolers in China.
Methods:
Three thousand eight hundred and twenty-one blood specimens from children aged 4 months to 6 years were collected and analyzed. Complete blood counts were determined by Sysmex XT-4000i Automated Hematology Analyzer. The nonparametric 2.5th to 97.5th percentile reference ranges were calculated according to CLSI EP28-A3c guideline.
Results:
Reference intervals for each blood cell parameter are determinded as follows: total WBC 4.86-12.1×109 /L for males and 4.73-12.3×109 /L for females; RBC 4.13-5.32×1012 /L for males and 4.08-5.24×1012 /L for females; HGB 109-145 g/L for males and 111-143 g/L for females; HCT 33.1-41.2% for males and 33.3-41.1% for females; MCH 23.5-29.7 pg for males and 24.6-30.0 pg for females; MCHC 320-365 g/L for males and 321-362 g/L for females; MCV 71.4-85.1 fL for males and 73.8-86.9 fL for females; RDW-SD 33.5-41.9 fL for males and 33.5-41.0 fL for females; RDW-CV 12.0-15.2% for males and 11.8-14.5% for females; PLT 181-475×109 /L for males and 179-456×109 /L for females; PCT 0.18-0.44% for males and 0.18-0.43% for females; MPV 8.20-11.6 fL for males and 8.20-11.5 fL for females; PDW 8.40-14.4 fL for males and 8.40-14.0 fL for females; P-LCR 12.0-36.6% for males and 11.8-35.6% for females.
Conclusions:
We established local complete blood count reference intervals for apparent healthy preschoolers in China. It is necessary to establishing region-specific reference intervals of complete blood count for preschoolers.
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