Lymphocyte extracellular matrix interactions. Induction of interferon by connective tissue components

Limiting dilution analysis was performed in the presence of interleukin 2 (IL-2) on lymphocytes isolated from the synovial fluid (SF) and peripheral blood (PB) of patients with rheumatoid arthritis (RA) and PB of normal donors. Clones of these 'spontaneously' IL-2-responsive cells from PB and SF were compared for their reactivity with components of the extracellular matrix (i.e. native or denatured type I or type II collagen and proteoglycan). It was determined that all clones from both PB and SF were activated to produce interferon (IFN) in the presence of any of the connective tissue components (CTC). Clones derived from normal PB behaved in a similar fashion but produced lower IFN-gamma levels. There was a synergy between the CTC and serum or plasma fibronectin, which was more apparent when soluble CTC were used as the stimuli rather than immobilized CTC. The fibronectin alone was unable to induce IFN-gamma production under any of the conditions tested (i.e. soluble or immobilized). These results demonstrate that clones of IL-2-responsive T cells can be activated by interactions with connective tissue components to produce IFN-gamma.