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Application of MALDI Biotyper to cyanobacterial profiling
Susumu Y Imanishi1, Takuma Nakayama1, Hirohiko Asukabe1
1Faculty of Pharmacy, Meijo University, Yagotoyama 150, Tempaku, Nagoya, 468-8503, Japan.
Rapid Communications in Mass Spectrometry : RCM
|November 19, 2016
Summary
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) rapidly profiles intact cyanobacteria. This method effectively differentiates harmful genera like Microcystis and Anabaena, aiding in bloom monitoring.
Area of Science:
- Microbiology
- Analytical Chemistry
- Environmental Science
Background:
- Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOFMS) is established for bacterial profiling.
- Previous applications of MALDI-MS for cyanobacterial profiling were limited.
- This study explores MALDI Biotyper analysis for rapid cyanobacterial profiling.
Purpose of the Study:
- To investigate the efficacy of MALDI Biotyper analysis for rapid profiling and differentiation of cyanobacteria.
- To assess the method's ability to distinguish between harmful genera such as Microcystis and Anabaena.
- To evaluate the potential for identifying cyanobacteria in natural water samples.
Main Methods:
- Cultured 22 cyanobacterial strains, including Microcystis and Anabaena species.
- Collected natural pond water samples containing cyanobacteria.
- Analyzed intact cyanobacterial cells using an Autoflex Speed MALDI-TOF mass spectrometer with Biotyper software.
- Utilized mass spectra (m/z 2000-20000) for hierarchical clustering and spectral library searching.
Main Results:
- Optimized MALDI-MS conditions yielded clear ion signals from cultured cyanobacterial cells.
- Hierarchical clustering successfully differentiated Microcystis and Anabaena strains into distinct clusters.
- Spectral library searching identified specific strains (Microcystis aeruginosa, Synechocystis sp.) even in mixed samples.
- Anabaena species were successfully identified in natural pond water samples.
Conclusions:
- MALDI-MS combined with Biotyper analysis is a viable method for cyanobacterial profiling.
- The spectral library size can be expanded to enhance monitoring of cyanobacteria in blooms.
- This technique offers a promising approach for identifying and differentiating cyanobacteria in environmental samples.
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