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Updated: Mar 11, 2026
High-resolution In Vivo Manual Segmentation Protocol for Human Hippocampal Subfields Using 3T Magnetic Resonance Imaging
Published on: November 10, 2015
A harmonized segmentation protocol for hippocampal and parahippocampal subregions: Why do we need one and what are
Laura E M Wisse1, Ana M Daugherty2, Rosanna K Olsen3
1Penn Image Computing and Science Laboratory, Department of Radiology, University of Pennsylvania, Philadelphia, USA.
Insights
Developing a standardized MRI segmentation protocol for hippocampal and parahippocampal subfields is crucial for consistent research across studies. This harmonized approach aims to improve data comparability in neuroscience and clinical research.
Area of Science:
- Neuroimaging
- Neuroanatomy
Background:
- High-resolution magnetic resonance imaging (MRI) enables in vivo study of hippocampal and parahippocampal subfields.
- Discrepant segmentation protocols hinder cross-study comparisons of neuroimaging data.
- Standardization is needed for reliable analysis of brain structure and function.
Purpose of the Study:
- To develop a harmonized manual segmentation protocol for hippocampal and parahippocampal subregions using high-resolution MRI.
- To address challenges in creating a standardized neuroimaging analysis protocol.
- To facilitate comparable research findings across diverse populations and studies.
Main Methods:
- International collaboration formed the Hippocampal Subfields Group.
- Focus on developing a versatile and reliable manual segmentation protocol.
- Addressing challenges including anatomical variability and protocol adoption.
Main Results:
- The commentary outlines key challenges in developing a harmonized protocol.
- Proposed solutions and working examples are discussed.
- The protocol aims to balance measurement reliability with anatomical validity.
Conclusions:
- A harmonized protocol will enable quantitatively comparable measurements across research labs.
- This standardization will facilitate the synthesis of findings from different neuroimaging studies.
- The protocol is expected to significantly impact the field of in vivo MRI research.
Abstract:
The advent of high-resolution magnetic resonance imaging (MRI) has enabled in vivo research in a variety of populations and diseases on the structure and function of hippocampal subfields and subdivisions of the parahippocampal gyrus. Because of the many extant and highly discrepant segmentation protocols, comparing results across studies is difficult. To overcome this barrier, the Hippocampal Subfields Group was formed as an international collaboration with the aim of developing a harmonized protocol for manual segmentation of hippocampal and parahippocampal subregions on high-resolution MRI. In this commentary we discuss the goals for this protocol and the associated key challenges involved in its development. These include differences among existing anatomical reference materials, striking the right balance between reliability of measurements and anatomical validity, and the development of a versatile protocol that can be adopted for the study of populations varying in age and health. The commentary outlines these key challenges, as well as the proposed solution of each, with concrete examples from our working plan. Finally, with two examples, we illustrate how the harmonized protocol, once completed, is expected to impact the field by producing measurements that are quantitatively comparable across labs and by facilitating the synthesis of findings across different studies. © 2016 Wiley Periodicals, Inc.
