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Rapid purification of squirrel monkey retrovirus-H major gag protein by high performance liquid chromatography
S Ikeda1, K Tsutsui, M Hatsushika
1Department of Biochemistry, Cancer Institute, Okayama University Medical School, Japan.
Acta Medica Okayama
|April 1, 1989
Abstract:
The major gag protein (p34) of squirrel monkey retrovirus-H was purified in one chromatographic step by anion-exchange high performance liquid chromatography. The virus in a crude fraction was disrupted with Brij 35 in the presence of three kinds of protease inhibitors. The soluble virus lysate was injected into a Polyanion SI column, and p34 was eluted with a linear salt gradient. The recovery of the protein was about 60%. The purified p34 was nearly homogenous as judged by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and silver staining.