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Updated: Mar 11, 2026
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RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
Published on: April 10, 2018
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Long Non-Coding RNAs: A Novel Paradigm for Toxicology
Joseph L Dempsey1, Julia Yue Cui2
1Department of Environmental and Occupational Health Sciences, University of Washington, Seattle, Washington 98105.
Summary
Long non-coding RNAs (lncRNAs) are key regulators in toxicology and human diseases. New technologies enable profiling lncRNAs to understand their roles in chemical exposures and health outcomes.
Area of Science:
- Molecular Biology
- Toxicology
- Genomics
Background:
- Long non-coding RNAs (lncRNAs) are crucial regulators of gene expression.
- Dysregulated lncRNAs are linked to diseases like cancer and Alzheimer's.
- lncRNAs play roles in intermediary metabolism and homeostasis.
Purpose of the Study:
- To review lncRNA biogenesis and functions.
- To highlight lncRNA roles in human diseases and toxicological responses.
- To evaluate methods for identifying aberrant lncRNA expression and interactions.
Main Methods:
- Genomic-scale profiling using next-generation sequencing.
- Computational algorithms for lncRNA characterization.
- Review of existing literature on lncRNAs in toxicology.
Main Results:
- lncRNAs regulate gene expression, chromatin remodeling, and mRNA processing.
- Chemical exposures impact lncRNA regulation.
- lncRNAs are implicated in various human diseases and toxicological responses.
Conclusions:
- lncRNAs are vital biomarkers and regulators in toxicology.
- Technological advancements facilitate lncRNA research.
- lncRNA tools can address fundamental toxicology questions.
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