Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Functional characterization of the uracil transporter from honeybee pathogen Paenibacillus larvae.

Microbial pathogenesis·2018
Same author

The solute transport and binding profile of a novel nucleobase cation symporter 2 from the honeybee pathogen <i>Paenibacillus larvae</i>.

FEBS open bio·2018
Same author

Complete Genome Sequences of Paenibacillus larvae Phages BN12, Dragolir, Kiel007, Leyra, Likha, Pagassa, PBL1c, and Tadhana.

Genome announcements·2018
Same author

The solute transport profile of two Aza-guanine transporters from the Honey bee pathogen Paenibacillus larvae.

FEMS microbiology letters·2018
Same author

Four Complete <i>Paenibacillus larvae</i> Genome Sequences.

Genome announcements·2017
Same author

Comparative analysis of Paenibacillus larvae genotypes isolated in Connecticut.

Archives of microbiology·2015

Related Experiment Video

Updated: Mar 11, 2026

A Visual Assay to Monitor T6SS-mediated Bacterial Competition
08:45

A Visual Assay to Monitor T6SS-mediated Bacterial Competition

Published on: March 20, 2013

16.2K

Functionality of Tn916 in Paenibacillus larvae.

Douglas W Dingman1

  • 1Department of Entomology, Connecticut Agricultural Experiment Station, 123 Huntington Street, P.O. Box 1106, New Haven, CT, 06504, USA. Douglas.Dingman@ct.gov.

Archives of Microbiology
|November 20, 2016
PubMed
Summary

The conjugative transposon Tn916 is functional in Paenibacillus larvae, enabling tetracycline and erythromycin resistance transfer. This genetic element shows stable maintenance and transferability within and between bacterial species.

Keywords:
American foulbroodConjugative transposonPaenibacillus larvaeTn916

More Related Videos

Functional Characterization of Carboxylesterases in Insecticide Resistant House Flies, Musca Domestica
08:17

Functional Characterization of Carboxylesterases in Insecticide Resistant House Flies, Musca Domestica

Published on: August 23, 2018

8.1K
Establishing the Minimal Bactericidal Concentration of an Antimicrobial Agent for Planktonic Cells MBC-P and Biofilm Cells MBC-B
06:36

Establishing the Minimal Bactericidal Concentration of an Antimicrobial Agent for Planktonic Cells MBC-P and Biofilm Cells MBC-B

Published on: January 2, 2014

20.7K

Related Experiment Videos

Last Updated: Mar 11, 2026

A Visual Assay to Monitor T6SS-mediated Bacterial Competition
08:45

A Visual Assay to Monitor T6SS-mediated Bacterial Competition

Published on: March 20, 2013

16.2K
Functional Characterization of Carboxylesterases in Insecticide Resistant House Flies, Musca Domestica
08:17

Functional Characterization of Carboxylesterases in Insecticide Resistant House Flies, Musca Domestica

Published on: August 23, 2018

8.1K
Establishing the Minimal Bactericidal Concentration of an Antimicrobial Agent for Planktonic Cells MBC-P and Biofilm Cells MBC-B
06:36

Establishing the Minimal Bactericidal Concentration of an Antimicrobial Agent for Planktonic Cells MBC-P and Biofilm Cells MBC-B

Published on: January 2, 2014

20.7K

Area of Science:

  • Microbiology
  • Bacterial Genetics
  • Horizontal Gene Transfer

Background:

  • Paenibacillus larvae is an important honeybee pathogen.
  • Conjugative transposons facilitate the spread of antibiotic resistance genes.
  • Understanding Tn916 mobility in P. larvae is crucial for controlling resistance.

Purpose of the Study:

  • To investigate the functionality and transferability of the conjugative transposon Tn916 in Paenibacillus larvae.
  • To assess the stability and insertion patterns of Tn916 and its derivative Tn916ΔE in P. larvae.
  • To determine the potential for interspecies and intraspecies transfer of Tn916 within Paenibacillus.

Main Methods:

  • Conjugative transfer experiments using defined donor and recipient strains.
  • Expression analysis of antibiotic resistance genes (tetracycline and erythromycin).
  • Southern hybridization for transposon insertion site analysis and copy number determination.

Main Results:

  • Tn916 demonstrated functional expression of tetracycline resistance and conjugative transfer in P. larvae.
  • Tn916ΔE also conferred erythromycin resistance, indicating its functionality.
  • Both interspecies and intraspecies transfer of Tn916 and Tn916ΔE were observed.
  • Southern hybridization revealed random chromosomal insertion of Tn916, with evidence of hot spots and predominantly single-copy insertions.
  • Multiple copies of Tn916 and Tn916ΔE could coexist in the P. larvae genome.
  • Tn916 and Tn916ΔE were stably maintained in P. larvae without antibiotic selection.

Conclusions:

  • The conjugative transposon Tn916 is a mobile genetic element capable of functional expression and transfer in Paenibacillus larvae.
  • Tn916 can transfer both within and between Paenibacillus species, contributing to the dissemination of antibiotic resistance.
  • The stable maintenance and potential for multiple-copy integration suggest Tn916 can persist and spread within bacterial populations.