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An Aptamer-based Sensor for Unchelated GadoliniumIII

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A highly Sensitive Turn-on Fluorescent Sensor for Ba²⁺ Based on G-Quadruplexes.

Lijun Xu¹, Yang Chen^1,2, Ruihua Zhang^1,2

¹Key Laboratory of Nano-Bio Interface, Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Suzhou, 215123, China.

Journal of Fluorescence

|November 20, 2016

Summary

A novel G-quadruplex fluorescent biosensor offers highly sensitive detection of barium ions (Ba²⁺). This turn-on method uses N-methyl mesoporphyrin IX (NMM) and shows great potential for real-time environmental monitoring.

Keywords:

Barium Fluorescence G-quadruplexes Label-free Sensors

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Area of Science:

Analytical Chemistry
Biochemistry
Environmental Science

Background:

Barium ions (Ba²⁺) are environmental contaminants requiring sensitive detection methods.
G-quadruplex DNA structures offer unique properties for biosensor development.
Fluorescent ligands like N-methyl mesoporphyrin IX (NMM) can signal molecular interactions.

Purpose of the Study:

To develop a novel, highly sensitive fluorescent biosensor for barium ion (Ba²⁺) detection.
To utilize G-quadruplex DNA structures for selective ion binding and signal generation.
To establish a rapid, economic, and on-site detection method for Ba²⁺.

Main Methods:

Construction of a G-quadruplex-based fluorescent biosensor using the AGRO100 DNA sequence.
Employing N-methyl mesoporphyrin IX (NMM) as a fluorescent probe.
Inducing G-quadruplex formation with Ba²⁺, leading to NMM fluorescence enhancement.

Main Results:

The biosensor exhibited a significant fluorescence enhancement (ca. 22-fold) upon addition of 1 μM Ba²⁺.
A linear response was observed in the range of 0-600 nM Ba²⁺, with a low detection limit of 4 nM.
The method demonstrated a turn-on response, speed, economic feasibility, and high signal-to-noise ratio.

Conclusions:

A novel and highly sensitive G-quadruplex-based fluorescent biosensor for Ba²⁺ detection was successfully developed.
The biosensor offers a rapid, economic, and environmentally friendly approach for on-site and real-time Ba²⁺ monitoring.
This method holds significant potential for environmental analysis and safety assessments.