Highlighting Gibberellins Accumulation Sites in Arabidopsis thaliana Root Using Fluorescently Labeled Gibberellins

The physical location of plant hormones is an important factor in maintaining their proper metabolism, perception, and mediated developmental responses. Thus, unveiling plant hormones dynamics at the molecule's level is essential for a comprehensive, detailed understanding of both their functions and the regulative mechanisms they are subjected to. Here, we describe the use of fluorescently labeled, bioactive gibberellins (GAs) to highlight the dynamic distribution and accumulation sites of bioactive GAs in Arabidopsis thaliana roots by confocal microscopy.