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PCR targeting IS6110 in diagnosing tuberculosis in children in comparison to MGIT culture

Rajeshwar Dayal1, Dipti Agarwal2, Harish Pathak3

  • 1Professor and Head, Department of Pediatrics, Sarojini Naidu Medical College, Agra, India.

The Indian Journal of Tuberculosis
|November 21, 2016
PubMed

Insights

Polymerase chain reaction (PCR) targeting IS6110 demonstrates superior sensitivity for diagnosing pediatric tuberculosis (TB), including extrapulmonary TB, compared to MGIT 960 culture. This molecular test offers improved diagnostic efficiency in children.

Area of Science:

  • Pediatric Infectious Diseases
  • Molecular Diagnostics
  • Microbiology

Background:

  • Diagnosing tuberculosis (TB) in children, particularly extrapulmonary TB (EPTB), presents significant challenges.
  • Accurate and timely diagnosis is crucial for effective treatment and disease control in pediatric populations.

Purpose of the Study:

  • To evaluate the diagnostic utility of polymerase chain reaction (PCR) targeting the IS6110 insertion sequence for TB detection in children.
  • To compare the performance of IS6110 PCR against MGIT 960 culture and conventional microscopy in pediatric TB cases.

Main Methods:

  • A study involving 142 pediatric patients (<15 years) with suspected TB (50 pulmonary, 92 extrapulmonary).
  • Clinical specimens were analyzed using Ziehl-Neelsen staining (ZN), MGIT 960 TB culture, and IS6110-targeted PCR.
  • Sensitivity and specificity of PCR were assessed and compared with MGIT culture results.

Main Results:

  • IS6110 PCR exhibited a significantly higher sensitivity (69.01%) than MGIT culture (47.41%) (p<0.05).
  • Sensitivity for PCR in extrapulmonary specimens (65.21%) was comparable to pulmonary specimens (76%), with no statistical significance (p>0.05).

Conclusions:

  • The diagnostic efficacy of IS6110 PCR was consistent across both pulmonary and extrapulmonary TB in children.
  • PCR utilizing the IS6110 primer demonstrated superior diagnostic efficiency compared to MGIT culture for pediatric TB.
Abstract

Keywords:
ChildrenDiagnosisMGITPCR IS6110Tuberculosis

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