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Resveratrol Inhibited Non-small Cell Lung Cancer Through Inhibiting STAT-3 Signaling

Xin Li1, Dan Wang1, Qing Chun Zhao1

  • 1Department of Lung Cancer Surgery, Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Heping District, Tianjin, China.

The American Journal of the Medical Sciences
|November 21, 2016
PubMed
Abstract

Insights

Resveratrol exhibits anticancer properties against non-small cell lung cancer by inhibiting STAT-3 signaling. This natural compound reduces cancer cell viability, proliferation, migration, and invasion while promoting apoptosis.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Resveratrol demonstrates potential anticancer effects, but its precise mechanisms, particularly in non-small cell lung cancer (NSCLC), require elucidation.
  • NSCLC represents a significant majority (80%) of all lung cancer cases, highlighting the need for effective therapeutic strategies.
  • This study investigates resveratrol's impact on A549 NSCLC cells in vitro.

Purpose of the Study:

  • To determine the anticancer effects of resveratrol on non-small cell lung cancer (NSCLC) cell line A549.
  • To elucidate the underlying molecular mechanisms, focusing on Signal Transducer and Activator of Transcription 3 (STAT-3) signaling.
  • To assess resveratrol's influence on cell viability, proliferation, apoptosis, migration, and invasion.

Main Methods:

  • Resveratrol's effects on A549 cell viability were assessed using sulphorhodamine B assays.
  • Cell proliferation and apoptosis were quantified via flow cytometry.
  • Cell migration and invasion were evaluated using Transwell chamber assays.
  • STAT-3 expression (mRNA and protein) was analyzed by real-time PCR and Western blot.
  • The functional role of STAT-3 was investigated using STAT-3 overexpressing vectors.

Main Results:

  • Resveratrol demonstrated dose- and time-dependent cytotoxicity against A549 cells.
  • Resveratrol significantly inhibited proliferation, migration, and invasion, while promoting apoptosis in a time-dependent manner (0-72 hours).
  • Resveratrol suppressed both mRNA and protein expression of STAT-3. Overexpression of STAT-3 partially or completely reversed resveratrol's inhibitory effects.

Conclusions:

  • The anticancer effects of resveratrol against non-small cell lung cancer cells are mediated through the modulation of STAT-3 signaling.
  • Targeting STAT-3 signaling represents a potential therapeutic strategy for NSCLC leveraging resveratrol.
Keywords:
A549 cellsApoptosisProliferationResveratrolSTAT-3

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