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Intratubular disinfection with tri-antibiotic and calcium hydroxide pastes
Thais Cristina Pereira1, Layla Reginna Silva Munhoz De Vasconcelos1, Marcia Sirlene Zardin Graeff1
1a Department of Dentistry, Endodontics and Dental Materials , Bauru Dental School, University of São Paulo , Bauru , SP , Brazil.
Objective:
The aim of this study was to compare the in vitro intradentinal antimicrobial ability of the calcium hydroxide and tri-antibiotic pastes.
Materials And Methods:
Standard bovine dentin tubes were sterilized and then infected with Enterococcus faecalis by a new contamination protocol of great depths of dentin. The specimens were filled with the medications, divided into two test-groups: calcium hydroxide (Group 1) and tri-antibiotic (Group 2) pastes. After 15 days, the teeth were evaluated by microbiological culture and confocal laser scanning microscopy (CLSM) with viability dye assay LIVE/DEAD inside dentinal tubules. In experiment of culture, the bacterial collection of the dentin fragments was done for counting the colony-forming units.
Results:
The tri-antibiotic paste had a slightly greater antimicrobial effect; however, there was no statistical difference between the groups.
Conclusions:
It was concluded that the tri-antibiotic paste and the calcium hydroxide paste exercise the same effect on intra-tubular decontamination against E. faecalis. So, due the multiples advantages, the calcium hydroxide paste can be the choice for dentinal decontamination in regenerative procedures.
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