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CRISPR-Based Technologies for the Manipulation of Eukaryotic Genomes
Alexis C Komor1, Ahmed H Badran1, David R Liu1
1Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA; Howard Hughes Medical Institute, Harvard University, Cambridge, MA 02138, USA; Broad Institute of MIT and Harvard, Cambridge, MA 02141, USA.
Cell
|November 22, 2016
Summary
CRISPR-Cas9 gene editing technology enables precise DNA modification in living cells. This review covers CRISPR tools for mammalian genome editing and their applications in research and medicine.
Area of Science:
- Molecular Biology
- Genetics
- Biotechnology
Background:
- CRISPR-Cas9 is an RNA-guided DNA endonuclease.
- It has driven significant advances in life sciences by enabling genome editing in living cells.
Purpose of the Study:
- To review CRISPR-based technologies for mammalian genome editing.
- To highlight their diverse applications and recent developments.
Main Methods:
- Summarizing CRISPR-based technologies.
- Describing recent advancements in CRISPR systems.
- Highlighting applications in basic research, biotechnology, and therapeutics.
Main Results:
- CRISPR technologies have been enhanced for generality, DNA specificity, and product selectivity.
- These advancements have facilitated remarkable progress in various scientific fields.
Conclusions:
- CRISPR-based technologies are powerful tools for mammalian genome editing.
- They have broad applications and continue to drive innovation in life sciences and medicine.
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