Temporal variation in the response of tumors to hyperoxia with breathing carbogen and oxygen

The effect of hyperoxygenation with carbogen (95% O₂ + 5% CO₂) and 100% oxygen inhalation on partial pressure of oxygen (pO₂) of radiation-induced fibrosarcoma (RIF-1) tumor was investigated. RIF-1 tumors were innoculated in C3H mice, and aggregates of oximetry probe, lithium phthalocyanine (LiPc), was implanted in each tumor. A baseline tumor pO₂ was measured by electron paramagnetic resonance (EPR) oximetry for 20 minutes in anesthetized mice breathing 30% O₂ and then the gas was switched to carbogen or 100 % oxygen for 60 minutes. These experiments were repeated for 10 days. RIF-1 tumors were hypoxic with a baseline tissue pO₂ of 6.2-8.3 mmHg in mice breathing 30% O₂. Carbogen and 100% oxygen significantly increased tumor pO₂ on days 1 to 5, with a maximal increase at approximately 32-45 minutes on each day. However, the extent of increase in pO₂ from the baseline declined significantly on day 5 and day 10. The results provide quantitative information on the effect of hyperoxic gas inhalation on tumor pO₂ over the course of 10 days. EPR oximetry can be effectively used to repeatedly monitor tumor pO₂ and test hyperoxic methods for potential clinical applications.