Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...

X-ray Crystallography

X-ray Crystallography

The size of the unit cell and the arrangement of atoms in a crystal may be determined from measurements of the diffraction of X-rays by the crystal, termed X-ray crystallography.
Diffraction
Diffraction is the change in the direction of travel experienced by an electromagnetic wave when it encounters a physical barrier whose dimensions are comparable to those of the wavelength of the light. X-rays are electromagnetic radiation with wavelengths about as long as the distance between neighboring...

Three-Dimensional Microscopy in Microbiology

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Texture tomography, a versatile framework to study crystalline texture in 3D.

IUCrJ·2024

Same author

Cellular and humoral immunogenicity of the COVID-19 vaccine and COVID-19 disease severity in individuals with immunodeficiency.

Frontiers in immunology·2023

Same author

Sub-pixel high-resolution imaging of high-energy x-rays inspired by sub-wavelength optical imaging.

Optics express·2021

Same author

Correlating dynamic strain and photoluminescence of solid-state defects with stroboscopic x-ray diffraction microscopy.

Nature communications·2019

Same author

Multimodal X-ray imaging of grain-level properties and performance in a polycrystalline solar cell.

Journal of synchrotron radiation·2019

Same author

Impact and mitigation of angular uncertainties in Bragg coherent x-ray diffraction imaging.

Scientific reports·2019

Same journal

Intranasal DNA nanocarrier vaccines with surface-patterned antigens enhance efficacy against respiratory syncytial virus.

Nature materials·2026

Same journal

An artificial neuromorphic interface for auditory restoration.

Nature materials·2026

Same journal

Seamless biointerfaces in devices.

Nature materials·2026

Same journal

Shaping the future of quantum technology.

Nature materials·2026

Same journal

Quantum tunnelling and leakage current across two-dimensional materials.

Nature materials·2026

Same journal

High-precision memristor-based computing.

Nature materials·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 11, 2026

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy

Published on: September 8, 2023

High-resolution three-dimensional structural microscopy by single-angle Bragg ptychography.

S O Hruszkewycz¹, M Allain², M V Holt³

¹Materials Science Division, Argonne National Laboratory, Argonne, Illinois 60439, USA.

Nature Materials

|November 22, 2016

Summary

This summary is machine-generated.

We developed 3D Bragg projection ptychography (3DBPP) for efficient nanoscale imaging of crystal lattice distortions. This method reconstructs 3D strain fields from 2D X-ray diffraction patterns, enabling detailed analysis of crystalline materials.

More Related Videos

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

Published on: May 27, 2008

Picometer-Precision Atomic Position Tracking through Electron Microscopy

Picometer-Precision Atomic Position Tracking through Electron Microscopy

Published on: July 3, 2021

Related Experiment Videos

Last Updated: Mar 11, 2026

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy

Author Spotlight: Non-Invasive Imaging of Complex Bio-Structures Using Polarization-Sensitive Two-Photon Microscopy

Published on: September 8, 2023

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

Non-invasive 3D-Visualization with Sub-micron Resolution Using Synchrotron-X-ray-tomography

Published on: May 27, 2008

Picometer-Precision Atomic Position Tracking through Electron Microscopy

Picometer-Precision Atomic Position Tracking through Electron Microscopy

Published on: July 3, 2021

Area of Science:

Materials Science
Crystallography
Nanotechnology

Background:

Coherent X-ray diffraction microscopy offers nanoscale 3D imaging of crystal lattice distortions.
Current methods face limitations due to stringent data measurement requirements.
Understanding nanoscale structure-property relationships under working conditions is crucial.

Purpose of the Study:

To introduce an efficient method for imaging 3D nanoscale lattice behavior and strain fields in crystalline materials.
To overcome limitations of existing X-ray diffraction microscopy techniques.
To enable high-resolution analysis of lattice distortions in functional materials.

Main Methods:

Developed 3D Bragg projection ptychography (3DBPP), a novel imaging methodology.
Utilized a series of 2D X-ray Bragg coherent intensity diffraction patterns measured at a single incident beam angle.
Reconstructed the 3D crystal volume by encoding structural information in reciprocal and real space.

Main Results:

Demonstrated the capability of 3DBPP for 3D image reconstruction of crystal volumes.
Successfully reconstructed lattice distortions in a component of a nanoelectronic prototype device.
Validated the analytical derivation and numerical simulation of the 3DBPP approach.

Conclusions:

3DBPP provides an efficient and effective approach for 3D nanoscale imaging of lattice distortions and strain fields.
The method overcomes previous data measurement limitations, broadening the applicability of X-ray diffraction microscopy.
This technique facilitates the study of structure-property relationships in crystalline materials at the nanoscale.